Anti Histone H3 T32ph - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Histone H3 T32ph Antibody

Catalog No.: CAC-CE-036A
Size: 100UL
[1 mg / mL]
Price: ¥58000
$774
antigen/source: Histone H3 T32ph
host: Rat
Application: Western Blot
Immunocytochemistry (cell)
Storage: -70C
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Immunogen: Human
Isotype: IgG2a
Clone: 6C7G12
Reacts with: Human
Mouse
Rat
Monkey
Hamster

Other:

 Background 
Histones are the main protein components of chromatin. To facilitate nuclear packaging and control of gene expression, DNA in chromatin is wound around nucleosome particles composed primarily of the Histones H2A, H2B, H3, and H4. Histone N-terminal regions (histone tails) protrude from the nucleosome core and are subject to a variety of reversible, regulated modifications (including acetlylation, phosphorylation, and methylation) influencing transcription and chromatin structure. How such modifications are regulated and how these modifications effect gene expression continues to be an area of intense interest and research. In such studies, chromatin immunoprecipitation (ChIP) is perhaps the most widely used experimental procedure. Due to the inherent variability and limited supply of polyclonal antibodies, well characterized monoclonal antibodies are preferred reagents for ChIP.
The versitile set of anti-histone monoclonal antibodies offered here are therefore highly valuable reagents to your lab's epigenetic toolbox.



 IMAGE1



The composition of Histone H3 peptides and the reactivity of Histone H3 T32ph antibody, 6C7G12.

 IMAGE2



Western blot analysis of the treated-cell extracts using Histone H3 T32ph antibody, 6C7G12. M: Add Nozodazole. I: After 12 hours from released Nocodazole. (Whole cell extracts)

 IMAGE3



Western blot analysis of the treated-cell extracts using Histone H3 T32ph antibody, 6C7G12. -: Before UV irradiation +: After UV irradiation (100J/m2, 2 hours) (Choromatin fractions)

 IMAGE4



Immunocytochemical analysis of eLa Cell using Histone H3 T32ph antibody, 6C7G12. (left : interphase, right : metaphase).
 
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