Anti Histone H3.1/H3.2 - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Histone H3.1/H3.2 Antibody

Catalog No.: CAC-CE-039A
Size: 100UL
[1 mg / mL]
Price: ¥58000
$774
antigen/source: Histone H3.1/3.2
host: Rat
Application: Western Blot
Storage: -70C
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Immunogen: Human
Isotype: IgG1
Clone: 6G3C7
Reacts with: Human
Mouse
Rat
Monkey
Hamster

Other:

 Background 
Nucleosomes are composed of four different histone proteins designated H2A, H2B, H3, and H4. In humans, five variants of histone H3 are reported: H3.1, H3.2, H3.3, H3t, and CENP-A. The two major Histone H3 variants, H3.1 and H3.3, are the main variants displaying distinct genomic localization patterns in eukaryotes. Deposition of Histone H3.1 is associated with DNA synthesis during DNA replication and possibly DNA repair, while Histone H3.3 is incorporated independently of DNA synthesis and is the predominant form of H3 found in non-dividing cells. Hence, these new Histone H3 variant monoclonal antibodies offer great utility for dissecting the functional significance of these H3 variants and the molecular mechanisms associatedwith their deposition.
Recently, it was shown that a genomic gene cluster regulating skeletal myogenesis is marked by H3.3 protein prior to cellular muscle formation and that H3.3 marking of this region enables myogenic gene activation (Ref. 2).These results suggest that monitoring H3.3 marking at specific loci may be useful in the prediction of cell fate. These H3.3 monoclonal antibodies are expected to be useful probes in the field of regenerative medicine.



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The composition of Histone H3 variants peptides and the reactivity using Histone H3.1 antibody, 6G3C7.

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Western blot analysis of recombinant protein and mammalian cell extracts using Histone H3.1 antibody, 6G3C7.

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Antibody specificity by competition peptide ELISA

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These H3 variant antibodies were essential tools in a first of kind study showing that differentiation specific genes are marked for lineage specific expression by the deposition of Histone H3.3 at the onset of differentiation signaling (Ref. 2).

Reference:

•  Hake SB. et al., PNAS. 103 6428-35 (2006)
•  Harada et al., EMBO J. 36 2994-3007 (2012)
 
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