LipoTrust™ EX Oligo for in vivo - Cosmo Bio Co.,Ltd.

Antibodies

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LipoTrust™ EX Oligo for in vivo

Catalog No.: CSR-LEO-10-EX
Size: 1ML
[10ƒĘmol]
Price: ¥144000
$1920
Storage: 4C
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Purpose: Transfection reagent for short oligonucleotides such as siRNA, antisense DNA or miRNA.
component: Lipo Trust™ Oligo for in vivo (Lyophilizate for 1ml) x 1
Instruction manual

Other:

 Background 
As most transfection reagents have little gene expression activity in serum-containing medium, use of serum-free medium is recommended. LipoTrust™ has enough gene expression activity in serum-containing medium as well, so that itcan be used in both type of experiments with or without serum either in vitro or in vivo.
Though general transfection reagents are known to be cytotoxic, LipoTrust™ causes much less cytotoxicity. Moreover, the product form, lyophilizate, makes it possible to choose from two types depend on your purpose. One is Coating Type, which is to be used by dissolving the empty LipoTrust™ with nucleic acid solution to reconstruct liposomal membrane without compromising effective nucleic acidretention. The other Lipoplex Type is the general type which is used by mixing the transfection reagent solution with nucleic acid solution.
LipoTrust™ EX Oligo is a proprietary cationic liposome formulation that facilitates highlyefficient delivery of short oligonucleotides such as siRNA, antisense DNA or miRNA into organs or tissues. You can easily prepare liposomal suspension for highly effective transfection with direct mixing of short oligonucleotides solution into lyophilized powder.
 Feature and advantages 
Higher knock-down efficiency is expected with low concentration of short oligonucleotide.
Transfection is available in the presence of serum.
Higher level transfection would be expected in vivo use withminimizing cytotoxicity.



 IMAGE1



As shown in the figure, more than 10% reduction of both cholesterol and LDL using siRNA mixed liposome were observed in comparison with that of siRNA-free liposome.

 IMAGE2



Both free siRNA and siRNA encapsulated in liposome were allowed to incubate in the bovine serum for more than 2 hours. Aliquots of serums were taken intermittently and respective RNAs were extracted .followed by siRNA contents analysis with HPLC. As shown in the Figure, free siRNA decomposed rapidly while as much as 50% of siRNA encapsulated in liposome were found to survive during 120 minutes incubation.

Reference:

•  T Kato et al., Gene Therapy, 1-9 (2010)
•  Robert Langer et al., Nature Review siRNA delivery 129-138
•  Kammei Rai et al., 100th AACR in April 19 (2009)
•  Hum. Gene Ther., 10,947-955 (1999)
•  J. Control.Release,62,269-277 (1999)
•  Biochim.Biophys.Acta,1467,419-430 (2000)
•  J.Control.Release,69,139-148(2000)
•  J.Biochem.,128,989-998(2000)
•  Pharm.Res.,19,377-381 (2002)
•  J.Biochem.,131.533-540 (2002)
•  Hypertension,40,148-154 (2002)
•  PHARM TECH JAPAN, 19,419-433 (2003)
•  Biotherapy,18,353-360 (2004)
 
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