Anti Pad - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Pad Antibody

Catalog No.: BAM-63-133-EX
Size: 100UL
Price: ¥35000
$467
antigen/source: Pad
host: Rabbit
Description: Fission yeast
Label: Unlabeled
Purity: Serum
Application: Western Blot
Immunoprecipitation
Storage: -20C
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Immunogen: Schizosaccharomyces pombe
Reacts with: Schizosaccharomyces pombe
Purity: Affinity Purified
Shape: Liquid
Purpose: Antibody for component of 26S proteasome

Other:

 Background 
Schizosaccharomyces pombe Pad1, a 35 kDa protein, is a component of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Transcription factor Pap1 is controlled by the functional interactionbetween the positive regulator Pad1 and negative regulator Crm1. Both proteins are essential for cell viability and for the maintenance of chromosome structure. Pad1 is also responsible for resistance to staurosporine, and other drugs such as cycloheximide and caffeine.
 Application 
Immunoblotting (dilution: 1/300~1/1000)
IP
Optimal dilutions/concentrations should be determined by the end user.



 IMAGE1



Fig.1 Fractions from sucrose gradient centrifugation of wild type S. pombe cells containing integrated Cut8-3HA were immunoblotted using antibodies Nuc2, Pad1 and HA (ref.2). Cut8 protein forms a broad peak around 4-15S (middle panel), distinct from the peak of 20S cyclosome (top panel) and 26S proteasome (bottom panel). Nuc2 and Pad1 are the subunits of cyclosome and proteasome, respectively.

Reference:

•  Shimanuki M et al.,. J Cell Sci 108: 569-579 (1995)PMID: 7769002
•  Tatebe H and Yanagida M., Curr Biol. 10:1329-1338 (2000) PMID: 11084332
•  Takeda K and Yanagida M., Cell 122:393-405 (2005) PMID: 16096059
 
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