Anti Rad18 - Cosmo Bio Co.,Ltd.

Antibodies

>

Anti Rad18 Antibody

Catalog No.: BAM-70-023-EX
Size: 100UG
Price: ¥34500
$460
antigen/source: Rad18
host: Rabbit
Label: Unlabeled
Purity: IgG fraction
Application: Western Blot
Immuno Fluorescence
Immunoprecipitation
Storage: -20C
@
Immunogen: Human
Reacts with: Human
Purity: Affinity Purified
Shape: Liquid

Other:

 Background 
The Rad6-Rad18 pair of genes plays a critical role in post-replication repair of damaged DNA. Rad6 protein functions as an E2 enzyme and Rad18 as an ubiquitine ligase (E3) which ubiquitinates PCNA. Rad18 recruits translesion DNA polymerases to damaged DNA (ref 1~3).
 Application 
WB (1000 fold dilution)
IF
IP
Optimal dilutions/concentrations should be determined by the end user



 IMAGE1



Fig.1 Indirect immunofluorescence staining of Rad18 protein in GM637 cells. Rad18 protein is stained as yellow dots in nuclei.

 IMAGE2



Fig.2 Identification of Rad18 protein in crude extract of A549 cells by Western blotting. The lower thick band is native Rad18 and the upper thin band is mono-ubiquitinated Rad18 protein (ref. 1)

 IMAGE3



Fig.3 Colocalization of Rad18 with PCNA on chromatin following UV irradiation. UV-induced colocalization of Rad18 (green) with PCNA (red). GM637 cells irradiated at 15/m2 were fixed with methnol 4h after UV irradiation and immunostained with anti-Rad18 antibody (#70-023) and anti-PCNA antibody and processed for double staining.

Reference:

•  Miyase S. et al., J. Biol. Chem. 280 515-524 (2005)
•  Tateishi S. et al., PNAS. 97 7927-7932 (2000)
•  Watanabe K. et al., EMBO. J. 23 3886-3896 (2004)
 
>