HIV-1Reverse transcriptase - Cosmo Bio Co.,Ltd.

Antibodies

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HIV-1Reverse transcriptase

Catalog No.: BAM-05-002-EX
Size: 1000UNIT
Price: ¥132000
$1760
Application: Western Blot
Enzyme Linked Immunosorbent Assay
Dot Blot
catalog info: Catalog 2012-p168
Storage: -20C
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Immunogen: Human Immunodeficiency Virus
Purity: >98% by SDS-PAGE
Purpose: [Application]
> It is highly effective for the screening of specific inhibitors for new AIDS treatment (3).
> Generally, Gag and Env proteins are employed as antigens for detecting anti-HIV-1 antibody. However, by using this enzyme in combination as an antigen, the detection will be more sensitive.
> Reverse transcriptases are used in the first step of RT-PCR reaction for converting RNA to DNA. The HIV-1 reverse transcriptase can also be applied for RT-PCR method.
> Standards for SDS-PAGE (Fig.1), Western blotting (Fig.2), Dot blotting, ELISA.
component: [Purity]
Over 90% by SDS-PAGE (CBB staining)
[Protein concentration]
0.37 mg / ml as measured by BCA method
[Specific activity]
10,000-20,000 units / mg
[Form]
50% glycerol, 40 mM Tris-HCl (pH8.3), 50 mM NaCl, 5 mM MgCl2 0.1% Triton X-100, 1 mM DTT

Other:

 Background 
HIV-1 reverse transcriptase is an RNA-dependent DNA polymerase of HIV-1 (AIDS virus), subtype B origin (1). It also has RNaseH activity and is an enzyme indispensable to the reproduction of AIDS virus. The product is uniquely over-expressed as a recombinant protein in E. coli by a patented method and highly purified (2). It is composed of two subunits (molecular weight of 66 kD and 51 kD), like the enzyme purified from AIDS virus particles (Fig 1).



 IMAGE1



Fig.1 Polyacrylamide gel electrophoresis of HIV-1 reverse transcriptase protein

 IMAGE2



Fig.2. Western blotting of functional recombinant full-length HIV-1 reverse transcriptase by using anti-HIV-1 Reverse Transriptase antibody (#65-001). 1; 40 ng / lane 2; 20 ng / lane 3; 4 ng / lane 4; 2 ng /lane Anti-HIV-1 RT antibody was used at 1/2,000 dilution. As second antibody, goat anti-rabbit IgG antibody conjugated with HRP was used at 1/5,000 dilution. ECL system was used.

Reference:

•  Adachi A. et al., J. Virol. 59, 284 (1986)
•  Saito A. et al., Microbiol. Immunol. 34:509-521 (1990)
•  Fischl MA. et al., N. Engl. J. Med. 317,185 (1987)
 
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