Anti Aggrecan (5G2) - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Aggrecan (5G2) Antibody

Catalog No.: CAC-PRPG-AG-M03
Size: 2ML
Price: ¥50000
$667
antigen/source: Aggrecan (5G2)
host: Mouse
Label: Unlabeled
Purity: Supernatant
Application: Western Blot
Immunohistochemistry
Enzyme Linked Immunosorbent Assay
Immunoprecipitation
Storage: -20C
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Immunogen: Human
Isotype: IgG1
Clone: 5G2
Reacts with: Human
Purpose: Western blotting: 1/10 - 1/30 (Distinct band at 260 kDa)
Immunoprecipitation: 1/5 - 1/10
Immunohistochemistry: 1/5 - 1/50 (paraffin-embedded)
ELISA: 1/10 - 1/150
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Other:

 Background 
Aggrecan is the major proteoglycan of cartilaginous ECMs, in particular the articular cartilage one, is a primary component of perineuronal nets of the CNS and is present in tendon, sclera and bone. While the precise function of aggrecan surrounding CNS neurons remains obscure, in articular cartilage it contributes to the creation of the hydrated gel structure of the ECM via its interaction with hyaluronan, link protein, CMPs, COMP and collagen type IX. Deletion of theaggrecan gene leads to early disturbances in chondrogenesis and brain formation.
[Confirmed Reactivity]
Human (not bovine, rat, chick or shark; other species not assayed)
Other species have not been tested.



 IMAGE1



Immunoblotting of a human articular cartilage aggrecan preparation following SDS-PAGE on 3-8% linear gradient gels under reducing conditions, prior to (Intact) and after combined chondroitinase ABC and keratanase II digestion (Digested). Immunostaining for aggrecan in human articular cartilage (B) and Aggrecan in human adult cerebral cortex (arrows point to neurons and perineuronal nets) (C).

Reference:

•  Virgintino D. et all.,J. Cell. Mol. Medicine 13 3151-3173 (2009)
 
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