Anti COMP (490D11) - Cosmo Bio Co.,Ltd.

Antibodies

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Anti COMP (490D11) Antibody

Catalog No.: CAC-PRPG-CP-M02
Size: 2ML
Price: ¥50000
$667
antigen/source: COMP (490D11)
host: Rat
Label: Unlabeled
Purity: Supernatant
Application: Western Blot
Immunohistochemistry
Enzyme Linked Immunosorbent Assay
Storage: -20C
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Immunogen: Human
Isotype: IgG1
Clone: 490D11
Reacts with: Human
Purpose: Western blotting: 1/20 - 1/70 (primary band at 97 kDa and a band at ~500 kDa under non-reducing conditions)
Immunohistochemistry: 1/20 - 1/75 (frozen and paraffin-embedded sections)
ELISA: 1/10 - 1/200
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Other:

 Background 
COMP - Cartilage oligomeric matrix protein - is a prominent multidomain glycoprotein of cartilage, accounting for up to 1% of the wet weight of articular tissues and having an approximate Mr of 97 kDa. COMP may also be found in tendon, bone (i.e. osteoblasts), ligament, certain smooth muscles and synovium. In the ECM COMP is present in a pentameric, disulfide-bonded complex of an Mr of about 550 kDa.
[Confirmed Reactivity]
Human, Bovine
Other species have not been tested.



 IMAGE1



(A) Western blotting of purified human COMP after SDS-PAGE on 4-15% gradient gels under reducing and non-reducing conditions. (B) and (C) Immunohistochemistry on normal articular cartilage. (D) ELISA on purified COMP [filled circles, mAb 490D11, empty circles mAb 484D1 (Catalogue number : PRPG-CP-M01), empty triangles, control antibody]

Reference:

•  Di Cesare P.E. et al., J. Orthopaed. Res. 18 713-720 (2000)
•  Di Cesare P.et al., Matrix Biol. 21 461-465 (2002)
 
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