Anti Versican / CSPG2 (4C5) - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Versican / CSPG2 (4C5) Antibody

Catalog No.: CAC-PRPG-VS-M02
Size: 2ML
Price: ¥50000
$667
antigen/source: Versican / CSPG2 (4C5)
host: Mouse
Label: Unlabeled
Purity: Supernatant
Application: Western Blot
IHC frozen section
Enzyme Linked Immunosorbent Assay
Storage: -20C
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Immunogen: Human
Isotype: IgM
Clone: 4C5
Reacts with: Human
Purpose: Western blotting: 1/20 - 1/40
Immunohistochemistry: 1/25 - 1/50 (PFA-frozen sections)
ELISA: 1/50 - 1/150
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Other:

 Background 
Versican, originally also known as PG-M, and encoded by the VCAN/CSPG2 gene, is a large extracellular matrix chondroitin sulfate proteoglycan ubiquitously expressed in interstitial matrices of the human body including that of brain. It was first isolated from the bovine aorta by Dick Heinegard's and Anders Malmstrom's groups (1982) and shortly after isolated from the chick embryonic limbs by Koji Kimata's group (1986). Cloning of the human VCAN/CSPG2 gene was accomplished in 1989by Zimmermann and Ruoslahti, who also cognated the name versican in recognition of its versatile modular structure.
 Cross reactivity 
Human, Bovine
Other species have not been tested.



 IMAGE1



(A) Immunoblotting of intact versican (mixture of V1 and V2 isoforms) prior to (Intact) and after combined chondroitinase ABC (Chase ABC) and endo-galactosidase-digestion (Digested) form. (B) Immunocytochemistry on cultured smooth muscle cells showing versican distribution in the ECM deposited by the cells. (C) Immunostaining of versican in normal human skin. (D) Immunostaining of versican distribution in the Bowman capsule of a normal human kidney (PFA-fixed frozen section).

Reference:

•  Mazzucato M. et al., FASEB J. 16 1903-1916 (2002)
•  Cattaruzza S. et al., J.Biol.Chem. 277 47626-47635 (2002)
•  Cattaruzza S. et al., Matrix Biol. 24 400-417 (2005)
 
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