Anti Centrin 1 - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Centrin 1 Antibody

Catalog No.: BAM-70-110-EX
Size: 100UG
Price: ¥35000
$467
antigen/source: Centrin 1
host: Rabbit
Purity: Affinity Purified
Application: Western Blot
Immuno Fluorescence
Storage: -20C
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Immunogen: Human
Reacts with: Human
Purity: Affinity Purified
Purpose: Western blotting: ~1/1000
Immunofluorescent staining: 1/200~1/500
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Other:

 Background 
Centrin 1 (human; 172 aa, MW; 19,570) plays a fundamental role in microtubuleorganizing center structure and function. Centrins, also known as caltractins, are a family of calcium-binding phosphoproteins found in the centrosome of eukaryotes. Centrins are present in the centrioles and pericentriolar lattice. Human centrin genes are
CETN1, CETN2 and CETN3. Centrins are required for duplication of centrioles. They may also play a role in severing of microtubules by causing calcium-mediated contraction.



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Fig.1 Identification of Centrin-1 in the crude extracts of human cells by Western blotting with this antibody HeLa cells (lane 1, 10 µg) and MCF-7 cells (lane 2, 20 µg). The antibody was used at 1/1000 dilution.

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Fig.2. Immunofluorescence staining of Centrin 1 protein in HeLa cells with anti-Centrin 1 antibody. Growing HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.25% Triton X-100. Anti-Centrin 1 antibody was used at 1/100 dilution. As 2nd antibody, goat anti-rabbit IgG conjugated with Alex 488 (red) at 1/1,000 dilution. DNA was stained with DAPI (blue).

Reference:

•  Salisbury JL., J Cell Physiol. 213:420-8 (2007) PubMed: 17694534
 
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