Anti RecA - Cosmo Bio Co.,Ltd.

Antibodies

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Anti RecA Antibody

Catalog No.: BAM-61-003-EX
Size: 50UG
Price: ¥31500
$420
antigen/source: RecA
host: Rabbit
Label: Unlabeled
Purity: IgG fraction
Application: Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
Immunoprecipitation
Chromatin Immunoprecipitation
catalog info: Catalog 2012-p84
Storage: 4C
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Immunogen: Escherichia coli
Reacts with: Escherichia coli

Other:

 Background 
E. coli RecA protein (352 aa, 38 kDa) plays critically important roles in homologous recombination, recombination repair and regulation of cellular responses to DNA damage (SOS response). RecA promotes auto-cleavage of LexA repressor byits coprotease activity after DNA damage, and induces many proteins related to DNA repair including RecA itself (1).

 Application 
1. Western blotting (1/3,000 dilution)

2. Immunoprecipitation (1/600 dilution)

3. Indirect immuno-fluorescent staining (assay dependent)
[Immunogen]
Highly purified full-length recombinant E. coli RecA protein

4. ELISA (assay dependent)



 IMAGE1



Fig 1. Western blot analysis of RecA protein in crude extract of E. coli.. Lane 1, Purified RecA 8 ng. Lane 2, Wild type. Lane 3, ⌂recA mutant. Lane 4, ⌂lexA mutant (SOS genes overexpressed). Antibody used at 1/3,000 dilution. Cell extracts, 2 µg

 IMAGE2



Fig 2. Immunoprecipitation of RecA protein from crudeextract of E. coli cells. Antibody 2 µg was adsorbed to proteinA magnetic beads to precipitate RecA protein from supernatant of sonic disrupted E. coli cells. The precipitate was analyzed by western blot.

Reference:

•  Friedberg EC et al., DNA Repair and Mutagenesis 2nd Ed. ASM Presss
 
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