Anti Neurocan - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Neurocan Antibody

Catalog No.: CAC-NU-07-002
Size: 200UL
[1 mg / mL]
Price: ¥50000
$667
antigen/source: Neurocan
host: Mouse
Description: Chondroitin sulfate proteoglycans purified from 10-day-old rat brain
Purity: Ig fraction - Protein G
Application: Western Blot
Enzyme Linked Immunosorbent Assay
Immunoprecipitation
IHC frozen section
IHC paraffin embedding section
Storage: -20C
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Immunogen: Rat
Isotype: IgG1
Clone: 1G2
Reacts with: Rat
Purpose: Western blotting: 1/10,000
- Immunohistochemistry: 1/100 (Frozen section), Available (Paraffin section)
- Immunoprecipitaion
- ELISA
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end

Other:

[BACKGROUND]
Neurocan is a nervous tissue-unique, secretory proteoglycan that carries predominantly chondroitin sulfate side chains. Its expression gradually decreases with the nervous tissue development. In the immature brain, neurocan exists inafull-length form with a 240 kDa-core glycoprotein, whereas it exists totally in proteolytic fragments of the NH2-terminal
half (neurocan-N) with a 130 kDa-core glycopeptide and the COOH-terminal half (neurocan-C) with a 150 kDa-core glycopeptide.Neurocan is implicated in the neural network formation, and is a susceptibility factor for bipolar disorder. This proteoglycan is upregulated in the lesion site of the central nervous system, and is a major component of the glial scar.
This antibodyrecognizes effectively the COOH-terminal half of rat neurocan core glycoprotein as well as the full length neurocan core glycoprotein.



 IMAGE1



Characterization of neurocan peptide pAb and neurocan mAb (clone 1G2) with samples obtained from 10-day-old rat brain. The PBS-soluble brain CSPG mixture of 10-day-old rats (lane 1) and the brain PBS-extract digested with chondroitinase ABC (lanes 2 and 3) were separated by SDS-PAGE. Neurocan peptide pAb recognized a very diffuse band with the same mobility as that recognized by eurocan mAb (clone 1G2). Monoclonal antibody (clone 1G2) recognized both 240 and 150 kDa core glycoproteins in the chondroitinase-digested sample. Polyclonal antibody recognized not only the 240 kDa core glycoprotein but also that with a molecular weight of 130 kDa. Reference : Neurochem. Int., 25 (1994) 425-431

 IMAGE2



Immunohistochemical staining using the monoclonal anti-neurocan antibody (clone 1G2) Cryostat sections of the retina from embryonic day 16 (E16) and newborn (P0) rats were stained with the monoclonal anti-neurocan antibody. Confocal images are shown together with sections stained with hematoxylin-eosin (HE). NFL, nerve fiber layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Reference: Invest. Ophthalmol. Vis. Sci., 40 (1999) 2350-2359.

Reference:

•  Oohira, A., et al., Neuroscience, 60, 145-157 (1994) PMID: 8052408
•  Matsui, F., et al., Neurochem. Int., 25, 425-431 (1994)
•  Watanabe, E., et al., Eur. J. Neurosci., 7, 547-554 (1995)
•  Fukuda, T., et al., J. Comp. Neurol., 382, 141-152 (1997)
•  Inatani, M., et al., Invest. Ophthalmol. Vis. Sci., 40, 2350-2359 (1999)
 
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