Anti Keratan Sulfate (5D4) - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Keratan Sulfate (5D4) Antibody

Catalog No.: CAC-PRPG-BC-M01
Size: 1ML
Price: ¥40000
$534
antigen/source: Keratan Sulfate
host: Mouse
Label: Unlabeled
Purity: Supernatant
Application: Western Blot
Immunohistochemistry
Storage: -20C
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Immunogen: Human
Isotype: IgG1
Clone: 5D4
Reacts with: All Species
Purpose: Western blotting: 1/100 (e.g. 50l to 5 ml with blocking buffer)
Immunohistochemistry: 1/20 (e.g. 20ul to 400ul with blocking buffer)
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Other:

 Background 
Monoclonal antibody 5D4 recognises oversulfated heptasaccharide epitopes containing 6-sulfated galactose adjacent with 6-sulfated N-acetyl-glucosamine in oligosaccharide segments of Keratan Sulfate glycosaminoglycan chains. Pre-digestion with Keratanase II removes these epitopes from Keratan Sulfate glycosaminoglycan chains . However, pre-digestion with Keratinase will not necessarily remove these Keratan Sulfate glycosaminoglycan chain epitopes.


Reference:

•  Schwend T. et al., Invest Opthamol Vis Sci. 53 8118-8137 (2012) PubMed: 23132805
•  Liles M. et al., Invest.Opthalmol. Vis. Sci. 51 1365-1372 (2010) PubMed: 19815728
•  Hayes AJ. et al., Methods 45 10-21 (2008) PubMed: 18442701
•  Hayes AJ. et al., J. Histochem. Cytochem. 55 853-866 (2007) PubMed: 17478447
•  Mehmet H. et al., Eur. J. Biochem. 157 385-391 (1986) PubMed: 2423332
•  Funderburgh JL. et al., Developmental Biology 116 267-277 (1986) PubMed: 2942429
•  Caterson B. et al., J. Biol. Chem. 258 8848-8854 (1983) PubMed: 6223038
 
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