Anti Chondroitin Sulfate(2B6) - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Chondroitin Sulfate(2B6) Antibody

Catalog No.: CAC-PRPG-BC-M02
Size: 1ML
Price: ¥40000
$534
antigen/source: Chondroitin Sulfate(2B6)
host: Mouse
Label: Unlabeled
Purity: Supernatant
Application: Western Blot
Immunohistochemistry
Enzyme Linked Immunosorbent Assay
Storage: -20C
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Immunogen: Bovine
Isotype: IgG1
Clone: 2B6
Reacts with: All Species
Purpose: Western blotting: 1/100 (e.g. 50 ul to 5 ml with blocking buffer)
Immunohistochemistry: 1/20 (e.g. 20l to 400l with blocking buffer).
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Other:

 Background 
Monoclonal antibody 2B6 recognises 4-sulfated unsaturated disaccharide neoepitopes (i.e. C-4-S "stubs") generated at the non-reducing terminal of Chondroitin Sulfate or Dermatan Sulfate glycosaminoglycan chains that have been
pre-digested with Chondroitinase ABC [see Figure 2; Caterson B (2012) Int. J. Exp. Pathol. 93: 1 - 10] but only Chondroitin Sulfate glycosaminoglycan chains pre-digested with Chondroitinase ACII or only Dermatan Sulfate glycosaminoglycan chains pre-digested with Chondroitinase B.


Reference:

•  Caterson B. et al., Int. J. of Exp. Path. 93 1-10 (2012) PubMed: 22264297
•  Hayes AJ. et al., Methods 45 10-21 (2008) PubMed: 18442701
•  Davies L. et al., Osteoarthritis & Cartilage 16 855-864 (2008) PubMed: 18222711
•  Hayes AJ. et al., J. Histochem. Cytochem. 55 853-866 (2007) PubMed: 17478447
•  Katz H. et al., J. Biol. Chem. 261 13393-13396 (1986) PubMed: 3531203
 
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