Anti Chondroitin Sulfate(1B5) - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Chondroitin Sulfate(1B5) Antibody

Catalog No.: CAC-PRPG-BC-M03
Size: 1ML
Price: ¥40000
$534
antigen/source: Chondroitin Sulfate(1B5)
host: Mouse
Label: Unlabeled
Purity: Supernatant
Application: Western Blot
Immunohistochemistry
Storage: -20C
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Immunogen: Rat
Isotype: IgG1
Clone: 1B5
Reacts with: All Species
Purpose: Western blotting: 1/100 (e.g. 50 ul to 5 ml with blocking buffer)
Immunohistochemistry: 1/20 (e.g. 20 ul to 400 ul with blocking buffer).
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Other:

 Background 
Monoclonal antibody 1B5 recognises unsulfated unsaturated disaccharide neoepitopes (i.e. C-0-S "stubs") generated at the non-reducing terminal of Chondroitin Sulfate glycosaminoglycan chains that have been pre-digested with either Chondroitinase ABC or Chondroitinase ACII [see Figure 2; Caterson B (2012) Int. J. Exp. Pathol. 93: 1-10].
[Reactivity of the antibody in undigested chondroitin]
An appropriate & valid gControlh for using mAbs 1B5, 2B6 & 3B3 is a no-chondroitinase treated sample. For mAbs 1B5 & 2B6 this should give a gblankh no-binding/no colour/no fluorescence result in Western Blots and IHC of sections. However, an exception is the use of mAb 3B3 [Please see Caterson (2012) published reference].
With this mAb 3B3, its use without chondroitinase treatment [denoted by us as 3B3(-)], can give a positive result when no chondroitinase treatment is used because it recognises an unusual non-reducing terminal epitope [i.e. not a chondroitinase-generated neoepitope that is designated by us as 3B3(+)] that consists of a terminal saturated glucuronic acid residue adjacent to a 6-sulphated N-acetyl-galactosamine residue in the native CS GAG chains [see Figure 2 in Caterson (2012)]. This reaction ofthe 3B3(-) mAb is seen when the mAb is used for both Western Blots & IHC sections. Examples of this use of 3B3(-) & 3B3(+) are provided in Caterson (2012) attached. Interestingly, expression of the 3B3(-) epitope is a biomarker for the presence of stem/progenitor cells in many tissues.
Caterson B. (2012). Chondroitin sulphate glycosaminoglycans: fun for some and confusion for others. Int. J. of Exp. Path. 93: 1 - 10


Reference:

•  Caterson B. et al., Int. J. of Exp. Path. 93 1-10 (2012) PubMed: 22264297
•  Hayes AJ. et al., Methods 45 10-21 (2008) PubMed: 18442701
•  Caterson B. et al., Federation Proceedings 44 386-393 (1985) PubMed: 2578417
•  Couchman JR. et al., Nature 307 650-652 (1984) PubMed: 6420711
 
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