Anti Chondroitin Sulfate(3B3) - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Chondroitin Sulfate(3B3) Antibody

Catalog No.: CAC-PRPG-BC-M04
Size: 1ML
Price: ¥40000
$534
antigen/source: Chondroitin Sulfate(3B3)
host: Mouse
Label: Unlabeled
Purity: Supernatant
Application: Immunohistochemistry
Enzyme Linked Immunosorbent Assay
Storage: -20C
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Immunogen: Rat
Isotype: IgM
Clone: 3B3
Reacts with: All Species
Purpose: Western blotting: 1/100 (e.g. 50 ul to 5 ml with blocking buffer)
Immunohistochemistry: 1/20 (e.g. 20 ul to 400 ul with blocking buffer).
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Other:

 Background 
Monoclonal antibody 3B3 recognises 6-sulfated unsaturated disaccharide neoepitopes (i.e. C-6-S "stubs") generated at the non-reducing terminal of Chondroitin Sulfate glycosaminoglycan chains that have been pre-digested with either Chondroitinase ABC or Chondroitinase ACII. Monoclonal antibody 3B3 also recognises a non-reducing end saturated disaccharide epitope in 'native' Chondroitin Sulfate glycosaminoglycan chains consisting of a terminal glucuronic acid adjacent to 6-sulfatedN-acetyl-galactosamine. The chondroitinase-generated neoepitope is often denoted as 3B3(+) and the 'native' terminal epitope as 3B3(-) in publications [see Figure 2; Caterson B (2012) Int. J. Exp. Pathol. 93: 1-10].


Reference:

•  Caterson B. et al., Int. J. of Exp. Path. 93 1-10 (2012) PubMed: 22264297
•  Hayes AJ. et al., Methods 45 10-21 (2008) PubMed: 18442701
•  Davies L. et al., Osteoarthritis & Cartilage 16 855-864 (2008) PubMed: 18222711
•  Caterson B. et al., J. Cell Sci. 97 411-417. (1990) PubMed: 1705939
•  Hayes AJ. et al., J. Histochem. Cytochem. 55 853-866. (2007) PubMed: 17478447
•  Caterson B. et al., Federation Proceedings 44 386-393 (1985) PubMed: 2578417
•  Couchman JR. et al., Nature 307: 650-652 (1984) PubMed: 6420711
 
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