Anti PsbO - Cosmo Bio Co.,Ltd.

Antibodies

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Anti PsbO Antibody

Catalog No.: AGR-AS05 092
Size: 100UL
Price: ¥112000
$1494
antigen/source: PsbO
host: Rabbit
Description: [Immunogen]N-terminal
Purity: Serum
Application: Western Blot
Immunohistochemistry
Immunoprecipitation
Storage: -20C
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Immunogen: Arabidopsis thaliana
Reacts with: Arabidopsis thaliana
Barley
Corn
Pisum sativum
Tobacco
Not Reacts with: Chlamydomonas reinhardtii
Shape: Lyophilized
Molecular Weight: 33 kDa
Purpose: Western blot: 1: 1000
Immunohistochemistry
Immunoprecipitation
Optimal dilutions/concentrations should be determined by the end user.



 IMAGE1



2 g of total protein from (1) Arabidopsis thaliana leaf, (2) Horderum vulgare leaf ), (3) Chlamydomonas reinhardtii total cell , (4) Synechococcus sp. 7942 total cell were all extracted with PEB (AS08 300) and separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).
 
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