Anti GOGAT - Cosmo Bio Co.,Ltd.

Antibodies

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Anti GOGAT Antibody

Catalog No.: AGR-AS07 242
Size: 200UL
Price: see also
#AGR-AS07242
Size 50
host: Rabbit
Purity: Serum
Application: Western Blot
Storage: -20C
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Immunogen: Arabidopsis thaliana
Reacts with: Arabidopsis thaliana
Corn
Phaseolus vulgaris
Tobacco
Shape: Lyophilized
Molecular Weight: 177 | 170-180 kDa depending upon the species
Purpose: Western blot: 1 : 1000
Optimal dilutions/concentrations should be determined by the end user.



 IMAGE1



20 g of total protein from (1) Spartina patens total cell extracted with Protein Extration Buffer, PEB (AS08300), (2) Spartina alterniflora total cell, extracted with PEB, (3) Miscanthus giganteus total cell extracted with PEB, (4) Zea mays total cell extracted with PEB, (5) Phaseolus vulgaris total cell extracted with PEB, (6) Arabidopsis thaliana total cell extracted with PEB, were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).
 
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