Anti 20S proteasome (GC3 α ) - Cosmo Bio Co.,Ltd.

Antibodies

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Anti 20S proteasome (GC3 α ) Antibody

Catalog No.: CAC-SZU-PS-M01
Size: 100UL
[1 mg / mL]
Price: ¥50000
$667
antigen/source: Purified 20S proteasome purified from goldfish ovary
host: Mouse
Label: Unlabeled
Purity: Affinity Purified
Application: Immunoelectron Microscopy
Western Blot
Immunohistochemistry
Storage: -20C
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Isotype: IgG1
Clone: GC3
Reacts with: Yeast
Fish
Frog
Rat
Human
Plant
Purpose: Immunoelectron microscopy: 1/100 - 1/200
Western blotting: 1/1000 - 1/2000
Immunohistochemistry: 1/200 - 1/1000
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Other:

 Background 
The 26S proteasome is an essential component of the ubiquitin-proteolytic pathway in eukaryotic cells and is responsible for the degradation of most cellular proteins. It is composed of a 20S proteasome as a catalytic core and regulatory particles at either end. The subunits of the 20S proteasome can be classified into two families, and . In eukaryotes, the 20S proteasome contains seven -type subunits and seven -type subunits. The fourteen subunits are arranged in four rings of seven and form an 7777 structure. This antibody recognizes several subunits of the 20S proteasome from all organisms tested, yeast to human. The advance of this antibody is application for immuno-electron microscopy.
[Source]
Serum free culture supernatant
[Purification]
Affinity purified by Protein G
[Concentration]
1 mg / mL
 Cross reactivity 
yeast, fish, frog, rat, human, plants




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Figure 1 Immunoblotting of the purified 26S proteasomes. 26S proteasomes were electrophoresed under denaturing conditions (12.0% gel) and stained with Coomassie Brilliant Blue (CBBR), or immunostained with antibodies (-20S, anti-Xenopus 20S proteasome polyclonal antibody; GC3; GC4/5; GC3; or 1-4D5) after electroblotting. Lanes I and M indicate 26S proteasomes from immature and mature oocytes, respectively. Protein bands that cross-reacted with GC4/5 (p25), GC3 (p30 and p31) and 1-4D5 (p62) are indicated. Molecular masses of standard proteins are indicated on the left. Reference : Eur J Biochem. 2000 Jan;267(1)97-103.

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Figure 2 Western blot analysis (A) Western blot analysis of proteins in rat testicular nuclei (Lanes1 and 3) and sperm heads (Lanes2 and 4). Lanes 1 and 2: pUP signals with mouse monoclonal antibody FK1. Lanes 3 and 4: 20S proteasome subunits detected by mouse monoclonal antibody GC3. Numbers, 31, 30, and 25 show molecular mass of each subunit, respectively. MM, molecular mass markers. (B) Western blot analysis of proteasomes partially purified from rat testes (Lane T) and liver (Lane L) with mouse monoclonal antibody to gold fish proteasome subunits (GC3). Reference: J Histochem Cytochem. 2007 Jun;55(6)585-95. Epub 2007 Feb 20.

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Figure 3 Immunofluorescence staining of rat epididymal sperm and human ejaculated sperm. (A) Smear preparation of rat sperm. (B) Human ejaculated sperm. Bar = 5 um.

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Figure 4 Immunoelectronmicroscopic localization of proteasome (A) Step 19 spermatids. Nuclear staining becomes weaker.Some gold particles are present in clear spots (arrows). Bar = 0.5 um (B) Nuclei of human ejaculated sperm Gold labeling is observed in the clear spots (short arrows) and in the vacuoles (long arrows) but not in the dense matrix. Bar = 0.5 um

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Reference:

◊  Haraguchi CM. et al.,, J Histochem Cytochem 55 585-595 (2007) PubMed: 17312012
•  Ohsaki Y. et al.,, Mol Biol Cell 17 2674-2683 (2006) PubMed: 16597703
◊  Tokumoto M. et al.,, Eur J Biochem 267 97-103 (2000) PubMed:10601855
 
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