Anti 20S proteasome (GC4/5) - Cosmo Bio Co.,Ltd.

Antibodies

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Anti 20S proteasome (GC4/5) Antibody

Catalog No.: CAC-SZU-PS-M03
Size: 100UL
[1 mg / mL]
Price: ¥50000
$667
antigen/source: Purified 20S proteasome purified from goldfish ovary
host: Mouse
Label: Unlabeled
Purity: Affinity Purified
Application: Western Blot
Storage: -20C
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Isotype: IgG2b
Clone: GC4/5
Reacts with: Yeast
Fish
Frog
Rat
Human
Plant
Purpose: Western blotting: 1/1000 - 1/2000
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Other:

 Background 
The 26S proteasome is an essential component of the ubiquitin-proteolytic pathway in eukaryotic cells and is responsible for the degradation of most cellular proteins. It is composed of a 20S proteasome as a catalytic core and regulatory particles at either end. The subunits of the 20S proteasome can be classified into two families, and . In eukaryotes, the 20S proteasome contains seven -type subunits and seven -type subunits. The fourteen subunits are arranged in fourrings of seven and form an 7777 structure.
This antibody recognizes 2 subunit of the 20S proteasome from all organisms tested, yeast to human. The advance of this antibody is application for immuno-electron microscopy.
[Source]
Serum free culture supernatant
[Purification]
Affinity purified by Protein G
[Concentration]
1 mg / mL
 Cross reactivity 
yeast, fish, frog, rat, human, plants



 IMAGE1



Figure 1 Immunoblotting of the purified 26S proteasomes 26S proteasomes were electrophoresed under denaturing conditions (12.0% gel) and stained with Coomassie Brilliant Blue (CBBR), or immunostained with antibodies (-20S, anti-Xenopus 20S proteasome polyclonal antibody; GC3; GC4/5; GC3; or 1-4D5) after electroblotting. Lanes I and M indicate 26S proteasomes from immature and mature oocytes, respectively. Protein bands that cross-reacted with GC4/5 (p25), GC3 (p30 and p31) and 1-4D5 (p62) are indicated. Molecular masses of standard proteins are indicated on the left. Reference : Eur J Biochem. 2000 Jan;267(1)97-103

 IMAGE2



Figure 2 Immunoelectronmicroscopic localization of proteasome 2 subunit

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Reference:

◊  Tokumoto T. et al.,, Exp Cell Res 247 313-319 (1999) PubMed: 10066358
◊  Wakata Y. et al.,, BMC Biochem 5 18 (2004) PubMed: 15603592
◊  Tokumoto M. et al.,, Eur J Biochem 267 97-103 (2000) PubMed: 10601855
 
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