GIP (Active) ELISA - Cosmo Bio Co.,Ltd.

Antibodies

>

GIP (Active) ELISA

Catalog No.: YII-YK251-EX
Size: 1KIT
Price: ¥79000
$1054
antigen/source: GIP (Active)
@
Immunogen: Rat
Purpose: Measurement of Rat GIP (Active) in plasma samples
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Antibody Coated Plate
2. Standard
3. HRP Labeled Antibody Solution
4. Enzyme Substrate Solution (TMB)
5. Stopping Solution
6. Buffer Solution
7. Washing Solution (concentrated)
8. Adhesive Foil

Other:

 Supplementary 
Sandwich
 Applicable sample 
rat plasma (EDTA-2Na + DPP-4
inhibitor)
 Background 
The incretin hormones, glucose-dependent insulinotropic polypeptide (GIP) and glucagons-like peptide-1 (GLP-1), are a group of gastrointestinal hormones that cause an increase in the amount of insulin released from the beta cells of the islets of Langerhans after ingestion of food.This ELISA kit has high specificity to rat GIP (1-42) active form and shows no crossreactivity to rat GIP (3-42)inactive form.
 Assay range 
3.9-250 pg/mL
[Kit Characteristics]
The assay runningtime: 3.5 hr.
Maximum measurable samples: 40 in duplicate
Test sample (size): Rat plasma (50 uL)
The kit can be used dividedly in strips.
Intra-assay CV (%):7.1-7.5
Inter-assay CV (%): 2.1-4.6


Reference:

•  Brown,J.C. et al., J.Physiol. 209 57-64 (1970)
•  Jornvall H et al., FEBS Lett. 123 205-210 (1981)
•  Moody,A.J. et al., Diabetologia 21 306 abstr (1981)
•  Carlquist M et al., Eur.J. Biochem. 145 573-577(1984)
•  Moody, A.J. et al., FEBS Lett. 172 142-148 (1984)
•  Takeda J, et al., Proc Natl Acad Sci USA. 84 7005-7008 (1987)
•  Pederson, R.A. in Gut Peptides: Biochemistry and Physiology, eds, Walsh, J.H.& Dockray, G.J. (Raven,New York), pp,217-260 (1994)
•  Rabinovitch, A. et al., Endocrinology 94 1139-1144 (1974)
•  Dupre,J. et al., J. Clin. Endocrinol. Metab. 37 826-828 (1973)
•  Elahi, D. et al., Am.J.Physiol. 237, E185-E191 (1979)
•  Krarup T et al., J Clin Endocrinol Metab. 56, 1306-12 (1983)
•  Naitoh R, et al., Biochem Biophys Res Commun. 376 21-5 (2008)
•  Miyawaki K et al., Proc Natl Acad Sci USA. 96 14843-4847 (1999)
•  Miyawaki K et al., Nat Med. 8 738-742 (2002)
•  Tsukiyama K et al., Mol Endocrinol. 20 1644-1651 (2006)
 
>