Anti CD9 for Exosome Isolation - Cosmo Bio Co.,Ltd.

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Anti CD9 for Exosome Isolation Antibody

Catalog No.: CAC-SHI-EXO-M01-EX
Size: 100UL
[1 mg / mL]
Price: see also
#CAC-SHIEXOM01
Size 100
antigen/source: CD9
host: Mouse
Description: [Immunogen]a.a.113-195
Label: Unlabeled
Purity: Ig fraction - Protein G
Application: Western Blot
Enzyme Linked Immunosorbent Assay
Immunoprecipitation
Storage: -70C
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Immunogen: Human
Isotype: IgG2b
Clone: 12A12
Reacts with: Human
Not Reacts with: Mouse/Rat
Purpose: Western blotting: 1/500 - 1/2,000 (0.5 - 2 ug/mL, non-reductive condition)
Immunoprecipitation: 1/200 - 1/1,000 (1- 5ug/mL)
* Experiment example : Serum Exosome
ELISA: 1/10,000 - 1/200,000 (5 ng/mL - 0.1 ug/mL)
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Other:

 Background 
CD9 is a cell surface glycoprotein which belongs to the tetraspanin superfamily. CD9 is known to complex with integrins and other transmembrane 4 superfamily proteins. It is found on the surface of exosomes. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation.
 Cross reactivity 
Human
Other species is not tested.



 IMAGE1



IP-WB of exosome in serum sample using Anti-CD9 antibody (12A12)

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Western blot analysis of CD9 in C32 cell Exosome with anti-CD9 (12A12) antibody

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ELISA data of C32 cell exosome with anti-CD9 (12A12-ALP) antibody (5 ng/mL)
 
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