Anti CD63 for Exosome Isolation - Cosmo Bio Co.,Ltd.

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Anti CD63 for Exosome Isolation Antibody

Catalog No.: CAC-SHI-EXO-M02-EX
Size: 100UL
[1 mg / mL]
Price: see also
#CAC-SHIEXOM02
Size 100
antigen/source: CD63
host: Mouse
Description: [Immunogen]a.a.104-202
Label: Unlabeled
Purity: Ig fraction - Protein G
Application: Western Blot
Immunoprecipitation
Storage: -70C
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Immunogen: Human
Isotype: IgG2a
Clone: 8A12
Reacts with: Human
Not Reacts with: Mouse/Rat
Purpose: Western blotting: 1/500 - 1/2,000i0.5 - 2ug/mL, non-reductive condition)
Immunoprecipitation: 1/200 - 1/1,000i1- 5ug/mL)
* Experiment example : Serum and Culture supernatant Exosome
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Other:

 Background 
CD63 (also known as LAMP-3, Melanoma-associated antigen ME491, TSPAN30, MLA1 and OMA81H) is a cell surface glycoprotein which belongs to the tetraspanin superfamily. CD63 is known to complex with integrins. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells.
 Cross reactivity 
Human
Other species is not tested.



 IMAGE1



IP-WB of exosome in serum sample using Anti-CD63 antibody (8A12)

 IMAGE2



Western blot analysis of CD63 in C32 cell Exosome with anti-CD63 (8A12) antibody

 IMAGE3



Immunoprecipitation in C32 cell (Melanoma cell line) Exosome
 
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