GenomONE™ -CF EX SeV-E (HVJ-E) 1 vial Cell Fusion Reagents - Cosmo Bio Co.,Ltd.

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GenomONE™ -CF EX SeV-E (HVJ-E) 1 vial Cell Fusion Reagents

Catalog No.: ISK-CF-001-EX
Size: 1SET
[for 10 hybridoma fusion experiments]
Price: ¥25000
$334
catalog info: Catalog 2012-p208
Storage: 4C
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Purpose: For hybridoma preparation and other cell fusion procedures.
component: Freeze-dried Sendai virus envelope (HVJ-E) - 1Vial (Approx. 0.26mL)
HVJ-E suspending buffer - 1Vial (0.5mL)
Cell fusion buffer (20~concentrate) - 1Vial (10 ml)
Instruction Manual - 1leaflet

Other:

 Background 
GenomONE™ -CF EX is a cell fusion kit comprised of Sendai virus envelope (SeV-E, HVJ Envelope, HVJ-E) and special buffers. It can be used with both adhering cells and suspension cells. Fusion of the same or different cell types can be completed in only 30 minutes.
 Feature and advantages 
GenomONE™ - CF EX produces more antibody secreting hybridomas than PEG. (following cell fusion, cells were grown in media containing hybridoma growth supplement as indicated).
Normal BALB/c mouse splenocytes (1x10^8 cells) not sensitized with antigen were fused to X63-Ag8.653 myeloma cells (1x10^7 cells) using GenomONE™ -CF EX or PEG1500. The fused cells obtained with each agent were inoculated onto five 96-well plates (Day 0). Beginning the following day, half of the culture medium (10%FBS/RPMI1640) was replaced with HAT medium at five points of time (Days 1, 2, 3, 5, and 8), and the growth of colonies in each well was assessed on Days 10 -11 to determine the hybridoma-positive rate (an indicator of efficiency of fusion). On Day 12, mouse antibody level (IgG + IgA + IgM) in the supernatant was measured by ELISA, to calculate the antibody production-positive rate. The effect of adding a commercially available hybridoma supplement to the medium after fusion was also assessed (supplement was also added to the HAT medium).
 Application 
1. Hybridoma preparation
Cell fusion involving PEG and electrofusion
Preparation of monoclonal antibodies
Fusion of antigen-sensitized B cells and myeloma cells
2. Fusion of suspension cells
3. Fusion of adherent cell and suspension cell
4. Application in premature chromosome condensation (PCC)
Detection of chromosomal damage and repair by fusion of different types of cells in different cell phases
5. Research on regenerative medicine and cytotherapy
Fusion of somatic cells and stem cells, etc.
6. Research on embryonic development/differentiation
Nuclear transfer (nuclear replacement)



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