GenomONE-Neo EX HVJ-E 40 vial(s) Transfection Reagents - Cosmo Bio Co.,Ltd.

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GenomONE-Neo EX HVJ-E 40 vial(s) Transfection Reagents

Catalog No.: ISK-GN-040-EX
Size: 1SET
Price: contact us
catalog info: Catalog 2012-p208
Storage: 4C
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Purpose: GenomONE™ -Neo EX is a non-viral reagent for transfection.

Other:

 Background 
HVJ (hemagglutinating virus of Japan) Envelope VECTOR KIT is a tool for transfection of molecules (plasmid DNAs, siRNAs, oligonucleotides, proteins, antibodies etc.) into cells and animal tissue by means of membrane fusion. The HVJenvelope, carrying the molecule to be transfected, is composed of a completely inactivated and purified HVJ (Sendai virus) while preserving the cell membrane-fusing capability of the envelope. GenomONE provides ready-to-use kits containing the HVJ envelope and auxiliary reagents (incorporation enhancer, incorporation reagent, introduction enhancer, buffer).
 Principle 
What is HVJ Envelope (HVJ-E)?
HVJ Envelope is a purified product prepared through complete inactivation of Sendai virus (HVJ*). It is a vesicle in which only the cell membrane-fusing capability of the envelope protein of Sendai virus is retained.
It is known that the HN protein in the tunica externa of the Sendai virus recognizes the receptor (possessing sialic acid atthe terminal of sugar chain) on the cell membrane and adsorbs it, leading to the induction of membrane fusion mediated by F protein (another component of the envelope). The genomic RNA of the Sendai virus contained in HVJ-E has been inactivated completely and has neither infective nor proliferative potentials in humans or experimental animals. HVJ-E can be used safely at ordinary laboratories, without requiring any special operations or facilities.
*HVJ:Hemagglutinating Virus of Japan.
Conventional non-viral transfection tools, including cationic lipids, are incorporated into cells through endocytosis which results in degradation of the transferred DNA by lysosomes. On the other hand, HVJ Envelope VECTOR resists degradation by lysosomes,making it easy to transfer the specified DNA. Therefore, HVJ Envelope VECTOR yields highly efficient gene expression. Sialic acid receptors, which are needed to trigger binding with HN protein, exist in almost all animal cells. Thus, HVJ EnvelopeVECTOR is useful for a wide range of targets.
[Features and Advantages]
1. Wide usability GenomONE HVJ Envelope VECTOR KIT is a highly flexible tool for transfecting a wide variety of molecules (plasmid DNAs, siRNAs, oligonucleotides, proteins,antibodies etc.) into cultured cells (adherent and non-adherent) and tissue. GenomONE is useful for transfecting sensitive primary cells and is further distinguished by its ability to deliver contents into live animals. Many literature citations areavailable for each GenomONE-Neo application.
2. Safety. Unlike cationic lipids, GenomONE-Neo delivers the molecule of interest into cells via membrane fusion, not by endocytosis where cargo may be degraded by lysosomal enzymes. Since GenomeONE-Neo isapurified HVJ (Sendai virus) product, prepared from virus particles completely inactivated for infectious ability and proliferative potential it is completely safe to use without special precautions.
3. Easy GenomONE provides ready-to-use kits containing the HVJ envelope and required auxiliary reagents (incorporation enhancer, incorporation reagent, introduction enhancer, and buffer). Suggested protocols for all major applications are included.



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Reference:

1. Kaneda, Y., et al.: Hemagglutinating virus of Japan (HVJ) envelope vector as a versatile gene delivery system. Molecular Therapy, 6, 219-226 (2002).
2)Kaneda, Y., et al.: New vector innovation for drug delivery: development of fusigenic non-viralparticles. Curr. Drug Targets, 4, 599-602 (2003).
 
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