Anti POGZ - Cosmo Bio Co.,Ltd.

Antibodies

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Anti POGZ Antibody

Catalog No.: BAM-70-112-EX
Size: 100UL
Price: ¥35000
$467
antigen/source: POGZ
host: Rabbit
Purity: Serum
Application: Western Blot
Immuno Fluorescence
Immunoprecipitation
Storage: -20C
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Immunogen: Human
Reacts with: Human
Mouse
Purity: Affinity Purified
Purpose: Western Blotting: 1/1,000
Immunofluorescece staining: 1/500
Immunoprecipitation: assay dependent
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Other:

 Background 
POGZ (pogo transposable element-derived protein with zinc finger domain; 1410 aa, 155 kDa) plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably throughits association with CBX5 (HP1 alpha) it plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms.
[Cross Reactivity] Human, Mouse
Not tested in other species



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Fig.1 Western blot of POGZ. Lane 1: Crude extract of HeLa cells transfected with control siRNA Lane 2: Crude extract of HeLa cells transfected with POGZ siRNA The antibody was used at 1/1,000

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Fig.2 Immuno-fluorescence staining of POGZ. Paraformaldehyde fixed HeLa cells were stained with Hoechst 33342 and anti-POGZ antibody. The antibody was used at 1/500 dilution. Scale bar, 10 um

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Fig. 3 Immuno-fluorescence staining of POGD on chromosomes. Chromosome spreads were prepared from HeLa cells and stained with Hoechst 33342 and anti-POGZ antibody. The antibody was used at 1/500 dilution. Scale bar, 10 um

Reference:

◊  Nozawa R.S et al., Nat. Cell Biol. 12 719-727 (2010)
 
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