Vitellogenin, ELISA - Cosmo Bio Co.,Ltd.

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Vitellogenin, ELISA

Vitellogenin; Vg

Catalog No.: KAL-KH075
Size: 1KIT
Price: [DISCONTINUED] Sorry!! This product is not available anymore.
antigen/source: Vitellogenin
catalog info: 2008 Japanes Assay kit Catalog-p839
Storage: -70C
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Immunogen: Killifish
Reacts with: Killifish
Measurement Range: 40 - 640 ng / mL
Purpose: Vitellogenin (Vg), a precursor of yolk protein, is a female-specific protein that is expressed only in the blood of higher vertebrate oviparous organisms. Vg is normally expressed in liver under regulation of estrogen (female sex hormome) during egg yolk
component: 1. Antibody-coated microtiter plate (8 wells x 12) 1 plate
2. The standard, Medaka Vg (64 ng / mL- ) 300 uL X 2
3. Dilution solution 30 mL X 2
4. Biotin conjugated Anti-medaka Vg antibody (X100) 80 uL X 1
5. HRP-conjugated streptavidin (X500) 20 uL X

Other:

 Supplementary 
Sandwich
 Applicable sample 
Killifish(Medaka) liver
[Other]
Dynamic range : 40 - 640 ng / mL
Background
In recent years the influence of Endocrin-Disrupting-Chemicals (EDC) upon living organisms has been one of the hot topics in environmental study. However, determination of the chemical compound species that have EDC-like influence has not yet been completed, and it is absolutely necessary to take actions to complete such a task.
Principle
This kit is based on the ELISA method which is used with specific anti- medaka polyclonal antibody. Anti- medaka polyclonal antibody is coated on the microplate, so that Vg in the sample and standard is captured. After reacting biotin-conjugated anti-medaka polyclonal antibody and HRP-conjugated streptavidin, the complex of ''coated-antibody - antigen - antibody'' is formed. Finally, you could measure the Vg concentration depend on the coloring strength which has developed with enzymatic reaction.


Reference:

1. The Effect of Estrogens, River Water, and Treated Wastewater on Vitellogenin Induction of Japanese Medaka Kiyoaki Kitamura, Kiyoshi Miyajima, Tadashi Higashitani, Norihide Nakada ,Koya Komori, Yutaka Suzuki, JAWRA, Volume 45, Issue 1, pages 22-34, February 2009
2. Reproductive effects and bioconcentration of 4-nonylphenol in medaka fish (Oryzias latipes). Ishibashi H, Hirano M, Matsumura N, Watanabe N, Takao Y, Arizono K., Chemosphere. 2006 Nov;65(6):1019-26.
3. Effects of pentachlorophenol on the reproduction of Japanese medaka (Oryzias latipes). Zha J, Wang Z, Schlenk D., Chem Biol Interact. 2006 May 15;161(1):26-36.
4. Development of on-site fish exposure system placed in water quality monitoring stations along a river. Higashitani T, Miyajima K, Nakada N, Yasojima M, Tanaka H, Suzuki Y., Water Sci Technol. 2005;52(12):275-82.
5. Validation of an Enzyme-Linked Immunosorbent Assay Method for Vitellogenin in the Medaka Norihisa Tatarazako, Masaaki Koshio, Hiroyuki Hori, Masatoshi Morita and Taisen Iguchi, JOURNAL OF HEALTH SCIENCE Vol. 50 (2004) , No. 3 301-308
6. Fish full life-cycle testing for androgen methyltestosterone on medaka (Oryzias latipes). Seki M, Yokota H, Matsubara H, Maeda M, Tadokoro H, Kobayashi K., Environ Toxicol Chem. 2004 Mar;23(3):774-81.
7. Effects of triclosan on the early life stages and reproduction of medaka Oryzias latipes and induction of hepatic vitellogenin. Ishibashi H, Matsumura N, Hirano M, Matsuoka M, Shiratsuchi H, Ishibashi Y, Takao Y, Arizono K., Aquat Toxicol. 2004 Apr 14;67(2):167-79.
8. Effects of 4-nonylphenol and 4-tert-octylphenol on sex differentiation and vitellogenin induction in medaka (Oryzias latipes). Seki M, Yokota H, Maeda M, Tadokoro H, Kobayashi K. Environ Toxicol Chem. 2003 Jul;22(7):1507-16.
 
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