Diacetylspermine (Urinary) ELISA
||2008 Japanes Assay kit Catalog-p716
||6.25 - 200 nM
||This kit is convenient to quantify amount of urinary diacetylspermine by using ELISA method.
1. Antigen coated microtiter plate, 96 wells 1 plate
2. Diacetylspermine standard 250 ul x 2
3. Antibody diluent 20mL x 1
4. Anti Diacetylspermine antibody concentrate(x 100) 80 ul x 1
5. HRP-anti Rabbit IgG Antibody concentrate(x 80) 60 ul x 1
6. OPD (o-phenylendiamine) tablets 2 tab.
7. Substrate solution 30 mL x 1
8. Stop solution 15 mL x 1
9. Wash buffer concentrate (x 20) 30 mL x 1
10. Dilution plate 1 plate
Polyamines are generally believed to function both in protein synthesis and DNA synthesis leading to control cell proliferation. In 1971, Russel firstly reported that total amount ofurinary polyamines elevated in cancer patients. And quantitative kit of urinary polyamines were already developed and utilized as a general biochemical examination. Recently two diacetyl-derivatives, N1, N12-diacetylspermine and N1, N8-diacetylspermidine, were found to be excreted in urine and form 0.6% and 1.4% of total polyamines respectively. Comparing urine of diseased person with urine of healthy person, some reports suggested the possibility that diacetyl-derivatives correlate to the status of disease more closely than total amount of polyamines.
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