Hexanoyl-Lys(HEL) ELISA kit - Cosmo Bio Co.,Ltd.

Antibodies

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Hexanoyl-Lys(HEL) ELISA kit

Catalog No.: NNS-KHL-700E-EX
Size: 96WELL
Price: ¥76800
$1024
antigen/source: Hexanoyl-lysine (HEL)
Description: Hexanoyl-lysine (HEL)
catalog info: Catalog 2012-p111
Storage: 4C
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Measurement Range: 2 - 700 nmol / L
Purpose: HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies. This HEL ELISA kit can be applied to urine, serum and cultured cells form human and animal.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. HEL Microtiter Plate Precoated with HEL (8x12 wells, Split Type) 1 Plate
2. Primary Antibody Monoclonal Antibody specific for HEL 1 Vial (7 mL )
3. Secondary Antibody HRP-Conjugated Anti Mouse IgG Antibody 1 Vial
4. Secondary Antibody Buffer Phosphate Buffered Saline 1 Vial (12 mL )
5. Chromogen 3,3',5,5'-Tetramethylbenzidine 1 Vial (250 UL )
6. Chromogen Buffer Hydrogen Peroxide/ Citrate-Phosphate Buffer 1 Vial (12 mL )
7. Washing Buffer (5X) Concentrated Phosphate Buffered Saline 2 Vials (25 mL X 2)
8. Stop Solution 1M Phosphoric acid 1 Vial (12 mL )
9. Standard A-F Bz-Gly-Hexanoyl-Lys 1 Vial each (500 UL )
10. Plate Seal Adhesive seal to prevent evaporation 2 Sheets

Other:

 Supplementary 
Competitive
 Applicable sample 
Urine, Serum, Culture Supernatant
[Other]
Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydessuch as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HELis formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.



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Reference:

•  N. Nakayama, et al., ACTA HEPATOLOGICA JAPONICA 31: 363-369 (1994)
•  Yamada A, et al., Biomedical Research 16 (2): 105-114 (1995)
 
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