FASTKIT ELISA Ver. II BUCKWHEAT
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||0.78 -50 ng / mL
||This kit is intended to measure buckwheat proteins in food products. This kit can measure standard buckwheat proteins in a solution at a concentration ranging from 0.78 to 50 ng/mL.
1. Antibody-immobilized microtiter plate (with a cover): A 96-well microtiter plate (8 wells x 12 lanes) x 1
2. Standard solution (50 ng / mL );manufactured by Nippon Gean Co., Ltd.: 1.8 mL x 1
3. Dilution buffer: 100 mL x 1
4. Biotin-conjugated antibody: 150 uL x 1
5. Streptavidin-conjugated enzyme (peroxidase): 150 uL x 1
6. Chromogenic substrate (TMB): 12 mL x 1
7. Solution to stop reaction (0.5N H2SO4): 12 mL x 1
8. Condensed wash solution (10-fold condensate): 100 mL x 1
9. Condensed extraction buffer 1. (20-fold condensate): 50 mL x 1
10. Condensed extraction buffer 2. (20-fold condensate): 50 mL x 1
11. Condensed extraction buffer 3. (20-fold condensate): 50 mL x 1
12. Instruction leaflet: 1 copy
With the amendment of Food Sanitation Law-related laws and regulations in April 2002, food manufacturers are now required to specify proteins derived from the designated five raw materials considered likely to induce food allergy (egg, milk, wheat, buckwheat, and peanut) on the product label for all food products containing at least a few ug/mL or ug/g of such proteins. To provide accurate protein information, it is important to perform an assay for the designated raw materials to confirm the content of protein and exclude possible raw material derived carry-over or unexpected contamination during the manufacturing process.
This product is an enzyme-linked immunosorbent assay (ELISA)-based measurement kit for buckwheat protein in foods. It has been confirmed to meet the ''Guidelines for Evaluating Test Methods for Food Products Containing Allergic Substances'' as specified in the PFSB/DFS Notification No. 1106001 fromthe Director of the Dept. of Food Safety, Pharmaceutical and Food Safety Bureau, Ministry of Health, Labour and Welfare dated November 6, 2002, ''Test methods for food products containing allergic substances'' (partially amended according to the PFSB/DFSNotification No. 1011002 dated October 11, 2005, PFSB/DFS Notification No. 0324001 dated March 24, 2006, and PFSB/DFS Notification No. 0622003 dated June 22, 2006 from the director of the same department).