MIF ELISA KIT, Human - Cosmo Bio Co.,Ltd.

Antibodies

>  

MIF ELISA KIT, Human

Catalog No.: SID-MIFHU100-EX
Size: 1KIT
Price: [DISCONTINUED] Sorry!! This product is not available anymore.
antigen/source: MIF
Application: Enzyme Linked Immunosorbent Assay
catalog info: Catalog 2012-p112
Storage: 4C
@
Immunogen: Human
Reacts with: Human
Sensitive: 1.6 ng / mL
Measurement Range: 0 - 100 ng/mL
Purpose: This kit can be used for quantitative determination of both natural MIF and recombinant MIF in various samples, such as serum, body fluid, buffered solution, cell culture medium, etc..
component: 1. Human MIF Standard 600L 1vial
2. Standard and AntibodyHRP Diluent Buffer 30mL 1bottle
3. MIF Antibody-coated wells 96wells 1plate
4. Peroxidase Conjugate 1mL 1vial
5. Stabilized Chromogen 12mL 1bottle
6. Stop Solution 20mL 1bottle
7. Wash Buffer Concentrate(20X) 30mL 1bottle
8. Accessories 2 adhesive strips

Other:

 Supplementary 
Sandwich
 Applicable sample 
serum, body fluid, buffered solution, cell culture medium
[Other]
Macrophage migration inhibitory factor (MIF) is the first T-cell-derived soluble lymphokine identified. It inhibits the random migration of macrophage out of capillary tubes and is profoundly involved in delayed-type hypersensitivity. Through the past few years, previously unrecognized properties of MIF have been discovered. In 1989, Human MIF cDNA was first cloned from activated-T-cells,demonstrating that this protein consists of 114 amino acid residues.The 25 amino acids from N-terminal of the rat MIF (13K protein) show 35% sequence homology with that of the rat glutathion S-transferase (GST) Yb subunit. This finding indicates that MIF is a unique protein linking the immune system with the detoxification system.
Recently, unexpected properties of this protein have been revealed.MIF works as an anterior pituitary-derived hormone, potentiating lethal endotoxaemia,and overrides the glucocorticoid-mediated suppression of inflammatory and immune responses. It was also found to play an essential role in the activation of T-cells after mitogenic or antigenic stimuli. On the other hand, it is reported that MIF mRNAexpression is correlated with differntiation of lens cells, suggesting the involvement of MIF in cellular proliferation and differentiation, and the presence of MIF protein was mostly limited to the basal layer. These results suggest that MIF may haveroles beyond the immune system.
Addition to these properties , MIF is expressed in a wide variety of organs, including the brain, kidney, and liver, and in a variety cells, suggesting its involvement in various pathophysiological phenomena beyondtheimmune system. Moreover the primary amino acid sequence identity between MIF molecules from various species is greater than 80%, suggesting MIF may be factor as homeostasis of cells. In this context, MIF should become a major target for the investigation of broad-spectrum pathophysiology and may be applicable for clinical therapeutic use in various disease in the near future, and MIF must be widely remarkable for its importance.


Reference:

1. Zhang JV, Ren PG et al: Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake. Science 310:996-999, 2005
2. Dun SL, Brailoiu GC et al: Distribution and biological activity of obestatin in the rat. J Endocrinol 191:1.10, 2006
3. Samson WK, White MM et al: Obestatin acts in brain to inhibit thirst. Am J Physol: Regulatory, Integrative and Comparative Physiolgy 292 (1): R637-643, 2007
4. Green BD, Irwin N and Flatt PR: Direct and indirect effects of obestatin peptides on food intake and the regulation of glucose homeostasis and insulin secretion in mice. Peptides 28:981-987, 2007
 
>