SMP30 [Gluconolactonase, GNL] WesternBlot & ImmunoStain Kit - Cosmo Bio Co.,Ltd.

Antibodies

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SMP30 [Gluconolactonase, GNL] WesternBlot & ImmunoStain Kit

Catalog No.: SML-ROIK01-EX
Size: 1KIT
Price: ¥68000
$907
antigen/source: SMP30
Application: IHC paraffin embedding section
Western Blot
catalog info: Catalog 2012-p147
Storage: -20C
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Immunogen: Rat
Reacts with: Rat
Mouse
Human
Purpose: Aging, Vitamin C and Lung Disease Research Kit
component: Antibody: Rabbit anti SMP30/GNL antibody 0.1 mL
10 mM Tris (pH 7.4), 0.14 M NaCl
This kit does not contain NaN3
Specimen: SMP30/GNL Knockout Mouse Liver 2 slides
Wild type Mouse Liver 2 slides
Tissue extract: SMP30/GNL Knockout Mouse Liver 30 PL
(Protein concentration 0.4 mg/mL)
Wild type Mouse Liver 30 PL
(Protein concentration 0.4 mg/mL)

Other:

 Background 
SMP30 (Senescence Marker Protein-30) is a 34-kDa protein whose tissue levels in the liver, kidney, and lung decrease with aging (1, 2). SMP30 expressed in multiple tissues including the liver, kidney, brain, lung, adrenal gland, stomach, ovary, uterus, testis and epidermis. Recently, SMP30 was identified as the lactone-hydrolyzing enzyme gluconolactonases (GNL) of animal species (3). GNL is a key enzyme which involve in vitamin C (L-ascorbic acid) biosynthesis. SMP30 knockout micedisplayed symptoms of scurvy when fed a vitamin C-deficient diet. Moreover, SMP30 protects mice lungs from oxidative stress associated with aging and smoking (4). Thus SMP30 is a key age-associated protein for aging, vitamin C and lung disease research.



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Western Blot Analysis Antibody: Rabbit anti SMP30/GNL antibody 0.1mL Specimen: SMP30/GNL Knockout Mouse Liver 2 slides, Wild type Mouse Liver 2 slides Tissue extract: SMP30/GNL Knockout Mouse Liver 30 mL (Protein concentration 0.4 mg/mL)

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Immunohistochemical staining

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Immunofluorescence staining Primary cultured mouse hepatocytes stained with SMP30/GNL antibody at 1:200 dilution. Nucleus and cytoplasm stained in green.

Reference:

•  Ishigami, A. et al., Senescence marker protein-30 knockout mouse liver are highly susceptible to TNF-alpha- and Fas-mediated apoptosis. Am. J. Pathol. 161 1273-1281 (2002)
•  Ishigami, A. et al., Nuclear localization of senescence marker protein-30 (SMP30) in cultured mouse hepatocytes and its homology to RNA polymerase. Biosci. Biotechnol. Biochem. 67 158-160 (2003)
•  Kondo, Y. et al., Senescence Marker Protein 30 Functions as Gluconolactonase in L-Ascorbic Acid Biosynthesis and Its Knockout Mice Are Prone to Scurvy. Proc. Nat. Acad. Sci. USA 103 5723-5728 (2006)
•  Sato, T. et al., Senescence Marker Protein-30 Protects Mice Lungs from Oxidative Stress, Aging and Smoking. Am. J. Respir. Crit. Care Med. 174 530-537 (2006)
 
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