Protein carbonyls western blot detection kit(15Blots) - Cosmo Bio Co.,Ltd.

Antibodies

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Protein carbonyls western blot detection kit(15Blots)

Catalog No.: SML-ROIK03-EX
Size: 1KIT
Price: [DISCONTINUED] Sorry!! This product is not available anymore.
antigen/source: DNP-KLH
host: Rabbit
Purity: IgG fraction
Application: Western Blot
catalog info: Catalog 2012-p145
Storage: 4C
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Purpose: For the specific detection of protein oxidation by ROS.
component: Antibody: Rabbit anti-DNP antibody 0.075 mL
10 mM Tris (pH 7.6), 0.14 M NaCl
This kit does not contain NaN3
The property of the antibody see below *
DNPH solution (shade the light) : 10X 2,4-Dinitrophenylhydrazine (DNPH) solution, 15 mL
Oxidized protein: oxidized BSA , soluble in SDS-PAGE sample buffer, 0.15 mL
[The property of the antibody]*
Rabbit Polyclonal Antibody
2,4-dinitrophenyl (DNP) IgG
Purified IgG Fraction
Rabbit anti-DNP IgG
Volume: 0.075 mL
Antigen : DNP-KLH
Host : Rabbit
Supplied As: IgG fraction purified from rabbit serum.
Prepared in 10 mM Tris (pH 7.6), 0.14 M NaCl.
Storage and Stability : antibody, DNPH solution, oxidized protein 4qC, 1 year

Other:

 Background 
Reactive oxygen species (ROS) are produced as a result of normal cell metabolism or by exposure to ionizing radiation, chemicals, or other environmental stress. ROS are well known to promote non-specific protein oxidation, with negative effects protein structure and function. Typical ROS-induced protein modifications include the transformation of lysine, arginine, proline, and threonine side chain amines into aminoacyl carbonyls. The chemical stability of these carbonyl derivatives allows their detection and quantification, providing a sensitive and reliable marker of ROS-mediated protein oxidation. In the central feature of this kit, carbonyl groups in tissue are first derivitized by reaction with 2,4-Dinitrophenylhydrazine (DNPH). DNPH-derivitized carbonyls are then detected using an anti-DNP specific antibody suitable for immunohistochemical procedures. The Protein Carbonyls Immunohistochemical Staining Kit is the first kit to enable detection of protein carbonyls by immunohistochemical staining. A detection kit for protein carbonyls in cell lysates by Western blotting with anti-DNP antibody is also available (Cat No.SML-ROIK03-EX).



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DNPH(+) : DNPH in 2 N HCl Left lane : oxidized BSA 0.1 Pg Right lane: mouse liver extract 5 Pg DNPH(-) : 2 N HCl Left lane: oxidized BSA 0.1 Pg Right lane: mouse liver extract 5 Pg CBB stain : proteins stained by Coomassie brilliant blue * DNP antibody at 1:2,000 dilution used.

Reference:

•  Nakamura A.et al.,J Biochem (Tokyo). 119 768-774 (1996)
•  Goto S.et al.,A critical evaluation. Age. 20 81-89 (1997)
•  Goto S.et al.,Mech Ageing Dev. 107 245-253 (1999)
•  Nakamura A.et al., Biochem Biophys Res Commun. 264, 580-583 (1999)
•  Robinson CE.et al.,Anal Biochem. 266, 48-57 (1999)
•  Sato T.et al.,Am J Respir Crit Care Med. 174 530-537 (2006)
◊  Drug Metab. Dispos., Oct 2010; 38: 1665 - 1672.
 
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