Blend Taq -Plus- - Cosmo Bio Co.,Ltd.

Antibodies

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Blend Taq -Plus-

Catalog No.: TYB-BTQ-201
Size: 1*250UNIT
Price: ¥24600
$328
catalog info: Catalog 2012-p210
Storage: -20C
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Purpose: For PCR
component: The provided reagents include the following components for 200 reactions:
Blend Taq® (2.5 U / ul) - 100 ul
10 x PCR Buffer for Blend Taq® - 1 ml
2 mM dNTPs - 1 ml
*This enzyme solution contains anti-Taq DNA polymerase antibodies.

Other:

 Background 
Blend Taq® and Blend Taq® -Plus- are highly efficient Taq-based DNA polymerases developed based on the Barns' method(1). This method uses a DNA polymerase which lacked 3'5' exonuclease (proofreading) activity (e.g., TaqDNA polymerase) and a small amount of an archaeal DNA polymerase with proofreading activity. Because the proofreading activity repairs misincorporated nucleotide bases causing the termination of the polymerase reaction, PCR with a 'mixed' enzyme solution enables highly efficient amplification.
Blend Taq® and Blend Taq® -Plus- generate dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA-cloning method.
 Application 
PCR
 Features 
Effective for the amplification of various targets from small starting template amounts.
The use of Hot Start technology with anti-Taq DNA polymerase antibodies results in highly efficient amplification.
The PCR error ratio of this enzyme is approximately 3-4 times less than that of Taq DNA polymerase.


Reference:

•  Barnes,W.M., Proc.Natl.Acad.Sci.USA, 91:2216-2220 (1994)
 
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