PerfectHyb Hybridization Solution - Cosmo Bio Co.,Ltd.

Antibodies

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PerfectHyb Hybridization Solution

Catalog No.: TYB-HYB-101
Size: 250ML
Price: ¥38700
$516
catalog info: Catalog 2012-p137
Storage: RT
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Purpose: Premixed hybridization solution for Northern and Southern Analyses containing a hybridization enhancer.

Other:

 Background 
PerfectHyb TM Hybridization Solution is a premixed buffer for hybridization. It has the following characteristics.
PerfectHyb TM Hybridization Solution is a premixed buffer for hybridization. It has the following characteristics.
¥Supports Northern and Southern blotting
¥Hybridization time can be cut
¥Allows analysis with both RI- and non-RI-labeled probes
¥Optimum buffer for analysis using a hybridization oven, as the temperature for hybridization is equal to that for probe cleaning
¥No need to add salmon sperm DNA, etc.
¥Low viscosity makes handling easy
[Applications]
Northern blotting
This product supports analysis using DNA probes, RNA probes and oligonucleotide probes. When used for pre-blotted type embrane*analysis, we recommend use of RI-labeled cDNA or oligonucleotide probes, taking into account background, rehybridization, etc.
Non-RI-labeled probes sometimes produce background signals depending on detection conditions. Experiments using such probes need to be done carefully, referring to the relevant instruction manuals.
* Good results have been obtained in analysis involving several manufacturers' preblotted membranes.
Southern blotting
We recommend use of DNA probes and oligonucleotide probes when using this product in Southern blotting. Non-RI-labeled probes sometimes produce background signals depending on detection conditions. Experiments using such probes need to be done carefully, referring to the relevant instruction manuals.
Hybridization enhancement
This product contains a hybridization enhancer, which allows for shortened hybridization. When used in Northern blotting, this product enhances signals compared to conventional methods.
[Additional material required]
The following are required to perform hybridization experiments using this product.
Reagents
¥Cleanser A:2 ~ SSC (pH 7.0), 0.1% SDS
¥Cleanser B*:0.1 ~ SSC (pH 7.0), 0.1% SDS
* Only necessary for Southern blotting or Northern blotting with RNA probes (cf. p.8 for details of reagent preparation)
Other equipment
¥Thermostat (preferably with a shaking function) or a hybridization oven
¥Heat sealer (unnecessary when an oven is used)
¥Hybridization bag (unnecessary when an oven is used)
¥X-ray film
[Features and advantages]
High Sensitivity
This solution contains a hybridization enhancer and generates a strong signal, enabling it effective detection of genes with low expression rates.Optimize Performance of a Broad Range of Applications. This solution can be used in Northern and Southern blot analysis. It is also ideal for non-RI analysis.
Rapid
Normal hybridization can be completed in 1-2 hours.
Easy to use
This solution does not develop crystal precipitation and has a low viscosity at normal temperatures, allowing simple handling. Addition of salmon sperm DNA is also not necessary. Moreover, it allows hybridization and washing procedures at the same temperature and is ideal for analysis using hybridizationovens.
[Recommendations]
Use for the mRNA Blotting Membrane
We recommend that RI-labeled cDNA probes or oligo probes be used in analysis of pre-blotting membranes in consideration of background.
Detection of the Gene Navigator#(R) cDNA Array System cDNA array
Use of PefectHyb#(R) Hybridization Solution -filter array- is recommended for detection of the above-mentioned product.



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Northern Blot Analysis Using PefectHyb

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Southern Blot Using PefectHyb
 
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