||For extraction of high purity genomes with magnetic slica beads
||Lysis & Binding Solution 500 ml
Washing Solution 500 ml x 2
Magnetic Beads 25 ml
This kit is for the extraction of high purity genomic DNA from biological samples such as blood and cultured cells. The recovered genomic DNA can be used in enzymatic reactions such as PCR. Since this kit employs the principle that magnetic silica beads bind genomic DNA present in a lysate solutions, deproteinization using harmful reagents such as phenol, ethanol precipitation, or high-speed centrifugation is not necessary, thus simplifying the extraction process. This kit is suitable for the MFX Series automatic nucleic acids extraction system and can also be used as a manual kit for B/F (solid-liquid) separation using a magnetic beads separation stand. When extracting genomic DNA from blood, whole blood can be used for DNA extraction. Leukocyte separation is not necessary as required by conventional methods.
Feature and advantages
Suitable for various kinds of samples
Allows the extraction of genomic DNA from samples such as whole blood, cultured animal cells, animal tissue, and mouse-tails.
Quick, Simple Extraction
Magnetic silica beads bind genomic DNA, allowing quick and simple extraction. Whole blood can be used when extracting genomic DNA from blood.
No Phenol or Chloroform Extraction
This kit doesnot require the use of harmful phenol or chloroform. Thus, no hazardous waste is produced.
Produces High Purity Genomic DNA
Genomic DNA extracted with this kit hardly contains any impurities such as RNA or proteins, allowing direct use in various experiments.
Genomic DNA extraction from whole blood, cultured animal cells, animal tissue, and mouse-tails.
The recovered genomic DNA can be used directly, mainly in PCR or other enzymatic reactions.
1. Examination of Yield and Purity
Genomic DNA was extracted from 100 ul of whole blood using this kit and others commercially available. The yield obtained with this kit was approximately equivalent to those obtained with the commercially available ones. With regard to purity,impurities such as proteins or RNA were more likely to have been present with use of the other commercially available kits (companies A and C), whereas such impurities were not detected with the use of this kit. The results show that use of this kitallows extraction of high purity genomic DNA from biological samples such as whole blood.
2. Example PCR Using Extracted DNA
PCR was carried out after extracting genomic DNA from 100 ul of whole blood collected from ten healthy, unrelated donors. Using 1/20 of the total amount of the extracted DNA, PCR was performed with Taq DNA polymerase with a single-locus probe of the VNTR regions of DNA, MCT 118, as a target. Approximately 1.4-2.2 ug of high purity genomic DNA with an A260/280 ratio between1.83 and 1.84 was obtained.
MCT118 is a repeated sequence of 16 bases, and by determining the number of these repeats, profiling can be performed. Specific bands for each individual were detected In this PCR with MCT118 as a target.
• B. Vogelstein et al., Proc. Natl. Acad. Sci. USA. 76 615-619 (1979)
• R. Boom, C. et al., J. Clin. Microbiol. 28 495-503 (1990)