MagExtractor -Plasmid- - Cosmo Bio Co.,Ltd.

Antibodies

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MagExtractor -Plasmid-

Catalog No.: TYB-NPK-301
Size: 500RXN
Price: ¥44100
$588
catalog info: Catalog 2012-p199
Storage: 4C
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Purpose: For plasmide etraction with magnetic silica beads.
component: Binding Solution 130 ml (with protein denaturant) x 2
Magnetic Beads I 16 ml
Magnetic Beads II 16 ml
Suspension Solution 80 ml
Lysis Solution I (with protein denaturant) 80 ml
Lysis Solution II (low concentration buffer) 20 ml
Neutralizing Solution 65 ml
Elution Solution 10 ml
Loading Dye 11 ml x 5

Other:

 Background 
This kit is for extraction of high purity plasmid DNA from bacteria such as E.coli. The recovered plasmid DNA can be used directly in enzymatic reactions including restriction endonuclease digestion, PCR sequencing and transformation.MAgnetic silica beads bind plasmid DNA present in a lysate solution. Deproteinization using harmful reagents such as phenol, or ethanol precipitation is not necessary. Furthermore, high-speed centrifugation is minimized, and is not necessary if the MFX Series automatic nucleic acids extraction system is used. This kit is suitable for the MFX series automatic nucleic acids extraction system and can also be used as a manual kit for B/F (solid-liquid) separation using a magnetic beads separation stand.
 Application 
Recovered plasmid DNA can be used directly in various enzymatic reactions and transformations.
1. Restriction Endonuclease Processing of the Extracted Plasmid
Plasmid was extracted from 1.2 ml of the solution cultured overnight of JM109 transformant harboring pUC18, using this kit and another commercially available kit. There was hardly any contamination by proteins, genomic DNA or RNA, and the plasmid DNA yield using this kit was equal to or higher than that obtainedwith the other commercially available kit. In addition, part of the obtained plasmid was digested with a restriction endonuclease, and the plasmid extracted with this kit as well as the other commercial available kit were confirmed to be completely digested. The result shows the use of this kit allows extraction of high purity plasmid from bacteria such as E.coli.
2. Sequencing Analysis of the Extracted Plasmid
An ABI PRISM 310 Genetic Analyzer was used to perform sequencing using 0.5 g ofpUC18 extracted with this kit. The sequence primer used was M13 Primer P8 (Code No. PRM-005) and the sequence kit was dRhodamine Terminator Cycle Sequencing FS Ready Reaction Kit (PE Applied Biosystems). The results show that plasmid extracted withthis kit is of sufficient quality to be used in auto-sequencing.



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Reference:

•  B. Vogelstein et al., Proc. Natl. Acad. Sci. USA. 76 615-619 (1979)
•  R. Boom, C. et al., J. Clin. Microbiol. 28 495-503 (1990)
 
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