Thermo T7 RNA Polymerase ((TT7)) - Cosmo Bio Co.,Ltd.

Antibodies

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Thermo T7 RNA Polymerase ((TT7))

Catalog No.: TYB-TRL-201
Size: 1*7500UNIT
Price: ¥16100
$215
catalog info: Catalog 2012-p210
Storage: -20C
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Purpose: To create RNA proble of high indication. To create precursor for RNA splicing reaction. To create mRNA with Cap analog as a primer. To create mRNA for in vitro Translation
component: Thermo T7 RNA polymerase (50U/ ul)
10X Buffer

Other:

 Background 
Thermo T7 RNA polymerase is a genetically modified T7 RNA polymerase exhibiting increased thermal stability. The optimum reaction temperature of this enzyme is around 50oC. The half-life of the enzyme at 50C is approximately 85 min.
 Features 
Exhibits greater specific activity than WT-enzyme at 37-50C.
 Application 
RNA probe preparation
RNA synthesis for in vitro translation
RNA synthesis for RNA splicing studies
Capped mRNA synthesis using a cap analogue


Reference:

•  K. Ishikawa et al., Nucl. Acids Res., 33; e112 (2005)
•  M. Itoh et al., Nucl. Acids. Res., 30; 5452-5464 (2002)
•  M. Chamberlin et al., J.Biol. Chem., 248: 2235(1973)
•  M. Chamberlin et al., J. Biol. Chem., 248: 2245(1973)
 
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