Leptin, ELISA (High Sensitivity)
||78.1-2,500 pg/mL (serum or plasma)
||This ELISA kit is used for quantitative determination of rat leptin in its serum and plasma samples. The kit is characterized for sensitive quantification, high specificity and no influences with other components in samples and needlessness of sample pretreatment. Rat leptin standard is recombinant product.
This kit shows 0.41% cross reactivity to human leptin, 0.1% to mouse leptin, and it has no cross reactivity with rat IL-1 α , IL-1 β , rat TNF- α , human TNF- α and other cytokines.
1. Antibody coated plate 1 plate (96 wells)
2. Standard Recombinant rat leptin (20 ng )
3. Specific antibody 1 bottle
4. HRP labeled antibody 1 bottle (12 mL )
5. Substrate buffer 1 bottle (26 mL )
6. OPD tablet 2 tablets
7. Stopping solution 1 bottle (12 mL )
8. Buffer solution 1 bottle (20 mL )
9. Washing solution 1bottle (50 mL )
10. Adhesive foil 4 sheets
Leptin, which is a product of ob gene, is a protein consisting of 167 amino acids and it is secreted from white adipose tissue. It is known that leptin acts on hypothalamus to decrease food intake and to reduce body weight, body fat, blood sugar and blood insulin in a healthy and an ob/ob mouse. Further, gene expression of neuropeptide Y (NPY) is suppressed by leptin. Recently, radioimmunoassay for leptin determination in human plasma has become available and leptin level in human patient group with obesity was found to increase in comparison with that of normal group. The level well correlate with body fat and these observations show clearly that leptin concentration in human plasma reflects the tissue fat weight. The measurement of plasma leptin may be an excellent index of obesity. Although rat leptin shows a high homology (96%) with mouse leptin, it is observed that substitution of several amino acid residues occurs at both end N- and Cterminal region between human and rat leptin. These findings have required urgently to develop highly sensitive immunoassay system specific to rat leptin.
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