Leptin, ELISA Kit - Cosmo Bio Co.,Ltd.



Leptin, ELISA Kit

Catalog No.: YII-YK052-EX
Size: 1KIT
[96 test]
Price: ¥60000
antigen/source: Leptin
Application: Enzyme Linked Immunosorbent Assay
catalog info: Catalog 2012-p112
Storage: 4C
Immunogen: Mouse
Reacts with: Mouse
Measurement Range: 0.313-20 ng/mL
Purpose: This ELISA kit is used for quantitative determination of mouse leptin in its serum samples. The kit is characterized by its sensitive quantification and high specificity. In addition, it is not influenced by other constituents in samples. Standard antigen, mouse leptin, in this kit is a recombinant product.
This ELISA kit is highly specific to mouse leptin. It shows 33.8 % crossreactivity to rat leptin and no crossreactivity to human leptin.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Antibody coated plate 1 plate (96 wells)
2. Standard antigen 1 vial (20 ng )
3. Labeled antibody solution 1 bottle (12 mL )
4. SA-HRP solution 1 bottle (12 mL )
5. Enzyme substrate solution 1 bottle (12 mL )
6. Reaction stopping solution 1 bottle (12 mL )
7. Buffer solution 1 bottle (15 mL )
8. Washing solution (concentrated) 1 bottle (50 mL )
9. Adhesive sheet 3 pieces


 Applicable sample 
Serum, Plasma
Leptin, which is a product of ob gene, is a protein consisting of 146 amino acids and it is secreted from white adipose tissue. It is known that leptin acts on hypothalamus to decrease food intake and reduce body weight, body fat, blood sugar and blood insulin in a healthy and an ob/ob mice. Further, gene expression of neuropeptide Y (NPY) is suppressed by this protein. Recently, radioimmunoassay for leptin determination inhuman plasma has become available and leptin level in human patient group with obesity was found to increase in comparison with that in normal group. The plasma level well correlated with body fat and these observations showed clearly that leptin concentration in human plasma reflects tissue fat weight. The measurement of leptin in plasma or serum may be a good index of obesity. Although mouse leptin shows a high homology (96%) with rat leptin in amino acid sequences, it is observed that substitution of quite a number of residues occurs between human and mouse leptin. These findings have urged us to develop highly sensitive immunoassay system specific to rat or mouse leptin.


•  Zanella E, Lunstra D, Wise T, Kinder J, Ford J. Biol Reprod Jan;60(1):115-118 (1999)
•  Colenbrander B, Van de Wiel DF, van Rossum-Kok CM, Wensing CJ. Biol Reprod Feb;26(1):105-109 (1982)
•  Wise T, Lunstra DD, Ford JJ. Biol Reprod Jan;54(1):146-153 (1996)
•  Kermabon AY, Prunier A, Djiane J, Salesse R. Biol Reprod Nov;53(5):1095-1102. (1995)
•  J Reprod Fertil Sep;75(1):17-28 (1985)
•  Guthrie HD, Bolt DJ. J Anim Sci Oct;57(4):993-1000 (1983)
•  Diekman MA, Trout WE, Anderson LL. J Anim Sci Jan;56(1):139-145 (1983)
•   Marshall J.C. Clinic in Endocrinol. Metab. 4:454 (1975)
•  Cohen K.L. Mtabolism 26:1165 (1977)
•  Rebar R.W., Erickson G.F. and Yen S.S.C. Fertil. Steril. 37:35 (1982)
•  Abraham G.E. Ed. Radioassay Systems in Clinic. Endocrinol. Marecel Dekker, Inc., New York (1981)
12. Uotila M., Ruoslahti E. and Engvall E. J. Immunol. Methods 42:11 (1981)