Insulin ELISA - Cosmo Bio Co.,Ltd.

Antibodies

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Insulin ELISA

Catalog No.: YII-YK060-EX
Size: 1KIT
Price: ¥50000
$667
antigen/source: Insulin
catalog info: Catalog 2012-p112
Storage: 4C
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Immunogen: Human
Reacts with: Human
Rabbit
Canine
Monkey
Measurement Range: 0.137 - 100 ng/ml
Purpose: This ELISA kit is used for quantitative determination of insulin in serum sample of human, rabbit and dog. The kit is characterized for sensitive quantification, high specificity and no influences with other components in samples and needlessness of sample pretreatment. Human insulin standard is recombinant product.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Antibody coated plate 1 plate (96 wells)
2. Standard 1 vial (100 ng )
3. Labeled antibody 1 bottle (12 mL )
4. SA-HRP solution 1 bottle (12 mL )
5. Substrate buffer 1 bottle (24 mL )
6. OPD tablet 2 tablets
7. Stopping solution 1 bottle (12 mL )
8. Buffer solution 1 bottle (25 mL )
9. Adhesive foil 3 sheets

Other:

 Supplementary 
Sandwich
 Applicable sample 
Serum
[Other]
This kit is a stable and convenient assay system for measurement of insulin (human, rabbit and dog) in serum samples. The processing of proinsulin, which occurs within the B cell,yieldsinsulin and C-peptide. Insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of insulin in blood is very important, and also provides valuable information to evaluate the pancreatic B cell function.
This kit for determination of insulin concentration (IR-insulin) in serum samples of human, rabbit and dog is based on a sandwich enzyme immunoassay by using combination of guinea pig anti human insulin antibody (coated on plate), recombinant insulin standard, biotinylated guinea pig anti human insulin antibody and HRP labeled streptoavidin (SA-HRP). Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of insulin is calculated.



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Performance Characteristics

Reference:

•  Bell GI, Pictet RL et al., Sequence of the human insulin gene. Nature 284: 26-32 (1980)
 
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