PYY EIA - Cosmo Bio Co.,Ltd.

Antibodies

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PYY EIA

Catalog No.: YII-YK081-EX
Size: 1KIT
Price: ¥76000
$1014
antigen/source: PYY
Label: Unlabeled
catalog info: Catalog 2012-p113
Storage: 4C
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Immunogen: Rat
Reacts with: Rat
Mouse
Measurement Range: 0.15 - 12.5 ng/mL
Purpose: This EIA kit is used for quantitative determination of rat PYY in rat plasma sample. The kit is characterized for sensitive quantification, high specificity and no influence with other components in plasma and needlessness of sample pre-treatment. PYY standard used in the kit is highly purified synthetic product (purity: higher than 98%).
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Antibody coated plate 1 plate (96 wells)
2. Rat PYY standard 1 vial (100 ng )
3. Labeled antigen 1 vial (1.5 ng )
4. PYY antibody 1 vial
5. SA-HRP solution 1 bottle (12 mL )
6. Substrate buffer 1 bottle (25 mL )
7. OPD tablet 2 tablets
8. Stopping solution 1 bottle (12 mL )
8. Stopping solution 1 bottle (12 mL )
9. Buffer solution 1 bottle (12 mL )
10. Washing solution 1 bottle (50 mL )
11. Adhesive foil 3 sheets

Other:

 Supplementary 
Competitive
 Applicable sample 
Serum, Plasma
[Other]
This enzyme immunoassay (EIA) kit is a stable and convenient assay system for peptide YY (PYY). PYY was isolated initially by Tatemoto et al. (1980 ) from the extract of pig duodenum and shown to be a polypeptide consisting of 36 amino acid residues. PYY is homologous to pancreatic polypeptide (PP) and neuropeptide Y (NPY). PYY is localized mainly in endocrine cells in the intestine (ileum, colon, and rectum). PYY shows an inhibitory action on contraction of the gastrointestinal tract and on secretion of pancreatic and gastric juice. PYY is released by taking diet. The PYY level decreases after resection of the intestine possibly are due to the decrease in number ofthe endocrine cells secreting PYY.&&The EIA kit is prepared by using synthetic rat PYY as standard antigen and biotinylated rat PYY as labeled antigen. The kit contains specific polyclonal antibody recognized to the amino acid sequence of rat PYY.


Reference:

•  Adrian, T.E., Smith, H.A., Calvert, S.A., Aynsley-Green, A. and Bloom, S.R. (1986), Pediatr Res.,20:1225-1227
•  Adrian T.E., Ferri, G.L., Bacarese-Hamilton, A.J., Fuessl, H.S., Polak, J.M. and Bloom, S.R.(1995), Gastroenterology, 89: 1070-1077.
•  El-Salhy, M., Grimelius, L., Wilander, E., Ryberg B., Terenius, L., Lundburg, J.M. and Tatemoto, K.(1983), Histochemistry, 77:15-23.
•  Greeley, G.H.Jr., Hashimoto, T., Izukura, M., Gomez, G., Jeng, J., Hill, F.L., Liuis, F.,and Thompson, J.C.(1989), Endocrinology, 125:1761-1765.
•  Greeley, G.H.Jr., Hill, F. L., Spannagel, A.and Thompson, J.C.(1987), Regul.Pept., 19, 365-372.
•  Gomez, G., Zhang, T., Rajaraman, S., Thakore, K.N., Yanaihara, N., Townsend C.M.Jr., Thompson, J.C. and Greeley, G.H.Jr.(1995), Am J.Physiol. 268, G71-G81.
•  Larhammar, D.(1996): Regul.Pept., 62, 1-11.
•  National Committee for Clinical Laboratory Standards Evaluation Protocols, SC1, (1989), Vallanova, PA: NCCLS
 
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