D-Serine Colorimetric Assay Kit - Cosmo Bio Co.,Ltd.

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D-Serine Colorimetric Assay Kit

Catalog No.: CSR-CT-DSC-K01E
Size: 1KIT
Price: ¥60000
$800
Storage: -20C
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Purpose: Measurement Kit for D-Serine in urine and food samples
component: Assay Buffer (11 mL ) x 2 vials
LDH Diluent Buffer (3 mL ) x 2 vials
10mM D-Serine Solution (2 mL ) x 2 vials
NADH Solution (200 uL ) x 2 vials
DsdSC Solution (110 uL ) x 1 vial
LDH Stock Solution (220 uL ) x 1 vial
96-wellplate x 1 plate (Non-strip plate)

Other:

 Background 
D-form amino acids have long been known as components of bacterial cell wall peptidoglycan layers. More recent developments in analytical technologies have demonstrated that D-amino acids are also present in mammals and display specificand important physiological activities. Particularly high levels of D-serine levels are present in brain tissue where D-serine functions as an important co-agonist of N-methyl-D-aspartate (NMDA) receptors (NMDR), involved in regulating higher brainfunction such as memory and learning. D-serine is suggested to play a role in neurodegeneration associated with diseases such as Alzheimer's disease (AD) and amyotrophic lateral sclerosis (ALS). High levels of free D-serine can also be found in humanurine although its significance is unclear.
D-serine is commonly assayed by HPLC or GC following conversion of D-serine to diastereomer derivatives. Such methods are time consuming, require expensive instrumentation, and are not suitable for processing large numbers of samples. The D-Serine Colorimetric Assay Kit employs the D-form specific enzyme D-serine dehydratase from Saccharomyces cerevisiae (DsdSC) enabling the quantitation of D-serine by spectrophotometric measurement.
 Advantages 
1. Enzymatic reaction with colorimetric detection for reading on standard UV/VIS absorbance microplate readers (340 nm).
2. Suitable for large numbers of samples.
3. Quantitative for D-serine detection. Detection range: 0.01 mM - 1 mM
[PrincipleofAssay]
DsdSC catalyzes the conversion of D-serine to pyruvate and ammonia. In the presence of lactate dehydrogenase (LDH), pyruvate is reduced to lactate with the concomitant oxidation of NADH to NAD. The reaction can be monitored by measuring thedecrease in absorbance at 340nm due to the oxidation of NADH. The D-serine concentration in unknown samples is determined by comparison with a standard curve.
 Sample 
Urine




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Figure 1: Principle of D-Serine Colorimetric Assay Detection

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Figure 2 : Overview of the assay procedure

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Figure 3 : D-Serine standard curve produced from assay data in Table 4

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Table 4 : Example assay data with D-serine standards

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Table5 : Example data for an assayed sample

Reference:

•  Tomokazu Ito, Kei Takahashi, Tomoko Naka, Hisashi Hemmi, Tohru Yoshimura (2007).
•  Tomokazu Ito, Hisashi Hemmi, Kunishige Kataoka, Yukio Mukai and Tohru Yoshimura (2008).
 
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