Cortisol ELISA - Cosmo Bio Co.,Ltd.

Antibodies

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Cortisol ELISA

Catalog No.: ENC-ERKB1004
Size: 1KIT
Price: [DISCONTINUED] Sorry!! This product is not available anymore.
antigen/source: Cortisol
Description: standard: native
catalog info: Catalog 2012-p110
Storage: 4C & -20C
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Immunogen: Bovine
Reacts with: Bovine
Sensitive: 0.5pg / mL
Measurement Range: 5-600ng/mL
Purpose: The Cortisol ELISA test is an immunoassay designed for the quantitative determination of cortisol in serum/plasma or urine samples. The test is intended for professional use as an aid in the diagnosis and monitoring of physiological/pathological conditions related to serum/plasma cortisol in Bovine and related species.
component: 1Microtiter wells coated with Cortisol specific antibody.
2. Enzyme labeled (HRP), 12mL
3. Cortisol Standards, 1 set ready to use. (Contains 0, 5, 10, 30, 100, 300, 600 ng/mL), 0.4ml/vial.
4. TMB Color Reagent (One-step ready to use), 12 mL
5. Stop Solution (2N HCl), 6 mL
6. 20x Wash Buffer, 20 mL.
7. Standard/Sample diluent, 25mL
8. Instructions

Other:

 Supplementary 
Sandwich
 Applicable sample 
Serum, Plasma, Urine
[Other]
The Cortisol Quantitative Test is based on a widely used immunoassay technique. A sample (serum/ plasma/urine) containing an unknown amount of cortisol to be assayed (unlabeled antigen) is added to a standard amount of a labeled derivative of the same substance (labeled antigen). The labeled and unlabeled antigens are then allowed to compete for high affinity binding sites on a limited number of antibodies coated on to the plate. After washing away the free antigen, the amount of labeled antigen in the sample is reversibly proportional to the concentration of the unlabeled antigen. The actual concentrations in unknown samples are obtained by means of a standard curve based on known concentrations of unlabeled antigen analyzed in parallel with the unknowns. In this kit an enzyme label is used. The biospecfic reaction takes place during 1 hour incubation. After washing away, substrate solution is added and the enzyme allowed to react for a fixed time before the reaction is terminated. Absorbencies are measured at 450 nm using ELISA plate reader. A standard curve is produced using values from 6 standards from which absorbency values for blank tubes have been subtracted. Results for unknown may be read directly from this standard curve using either manual calculation or by a suitable computer program.
This kit is suitable for the direct measurement of cortisol in serum/plasma/urine samples. Allsamples to be assayed must be diluted with sample dilution buffer (ed. 1part with 4 parts of sample buffer ; 0.05 mL + 0.2ItmL). It may also be used following an extraction procedure for assaying tissue cortisol (call for details of the procedure). Note: The cortisol levels should be established in your laboratory using your own set of samples and standards and good laboratory practice should be employed where applicable.


Reference:

1. BONDY PK. The adrenal cortex, in Randy PT, Rosenberg LE., Metabolic control and disease (8 ed) 1980, WB Sanders, Philadelphia, p1427-1499.
2. Lambert A Clinical Endocrinology 1974, Springer-Verlag New York, p299-305
3. Veosei P, Glucocorticoids, Aldosterone, corticosterone, Compound S in Jaffe BM, Behrman HR 1974 in Methods in Radioimmuno assay p393-411.
4. Spark R 1971, Simplified assessment of pituitary-adrenal reserve Annals of internal Med. 75 p75-717
 
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