Estradiol ELISA - Cosmo Bio Co.,Ltd.

Antibodies

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Estradiol ELISA

Catalog No.: ENC-ERKB1005
Size: 1KIT
Price: ¥125000
$1667
antigen/source: Estradiol
Description: standard: native
catalog info: Japanese Catalog No.14-p1456
Storage: 4C & -20C
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Immunogen: Bovine
Reacts with: Bovine
Sensitive: 0.5 pg / mL
Purpose: The Microwell Estradiol ELISA TEST is an enzyme immunoassay system for quantitative determination of 17 beta Estradiol levels in bovine and related species serum. The test is intended for professional use as research tool in monitoring of conditions related to serum/plasma levels of Estradiol. The test kit has been designed to be used by a trained, skilled laboratory professional only.
component: 1. Microtiter wells 96, coated with second antibody.
2. Enzyme Conjugate solution, 12mL.
3.TMB Color Reagent, 12 mL
4. Stopping Solution (2N HCL), 6mL
5. 20 X Wash Buffer, 20 mL.
6.Sample diluent, 20mL
7. E2 Standard Set: 0, 10, 25, 100, 500 and 2500, 10,000 pg/ml/ 0.5ml/Vial
8. Quality control set QC1, (~100pg/mL) and QC2, (~500 pg/mL)

Other:

 Supplementary 
Sandwich
 Applicable sample 
Serum, Plasma
[Other]
The E2 quantitative test is based on a solid-phase enzyme immunoassay based on competitive binding method. A sample (serum/ plasma/urine) containing an unknown amount of E2 to be assayed (unlabeled antigen) is added to a standard amount of a conjugated E2 (labeled antigen). The labeled and unlabeled antigens are then allowed to compete for high affinity binding sites of E2 antibody on a coated plate. After washing away the free antigen, the amount of labeled antigen in the sample is reversibly proportional to the concentration of the unlabeled antigen. The actual concentrations in unknown samples are obtained by means of a standard curve based on known concentrations of unlabeled antigen analyzed in parallel with the unknowns. After washing, substrate solution is added and the enzyme is allowed to react for a fixed time before the reaction is terminated. Absorbencies are measured at 450 nm using ELISA plate reader. A standard curve is produced using values from 5 standards from which absorbency values for blank tubes have been subtracted. Results for unknown may be read directly from this standard curve using either manual calculation or by a suitable computer program. This kit is suitable for the direct measurement of E2 in serum samples


Reference:

1. Webster J., 1987 understanding the dairy cow. BSP professional Books. Oxford p64-68, 300-321, and 343-344.
2. Robinson T.J. 1977 Reproduction in cattle ''In reproduction in Domestic animals'' 3rd ed.HH.Cole & PT Cupps editors, p439-442
3. BulmanDC.& Lamming GE. 1978 Milk progesterone levels interrelation to conception, repeat breeding and factors influencing acyclicity in dairy cows. J Reprod Fertil.54, 447-458
4. Ruiz FJ et al. 1992 Cost benefit evaluation of on-farm milk progesterone testing to monitor return to cyclicity and to classify ovarian cysts. J Dairy Sci 1992 75(4), 1036-1043
5. Autrere MB and Benson H 1976 Progesterone: an overview and recent advances Jour Par Sci 65, (6)783-800
6. Chattoraj SC 1976 Endocrine functioninFundamentals of Clinical Chemistry, NW Tietz eds.,WB Saunders, Chap 13, 699-823
 
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