Estradiol ELISA - Cosmo Bio Co.,Ltd.

Antibodies

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Estradiol ELISA

Catalog No.: ENC-ERKC2005
Size: 1KIT
Price: ¥125000
$1667
antigen/source: Estradiol
Description: standard: native
catalog info: Catalog 2012-p111
Storage: 4C & -20C
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Immunogen: Canine
Reacts with: Canine
Sensitive: 5 pg / mL
Purpose: The Microwell Estradiol ELISA is an enzyme immunoassay system for quantitative determination of Estradiol levels in Canin serum. The test is intended for professional use as research tool in monitoring of conditions related to serum/plasma levels of Estradiol. The test kit is designed for a trained, skilled laboratory professional only.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Microtiter wells 96, coated with second antibody.
2. Rabbit anti E2 antibody 7.0 mL
3. Enzyme Conjugate solution, 12 mL.
4. E2 Standard set: 0,10, 30,100, 300, 1200, 5000pg/mL. (+ QC1(range 50 pg / ml) and QC2 (range 300 pg / ml))
5. TMB Color Reagent, 12 mL
6. Stopping Solution (2N HCL), 6 mL
7. 20 X Wash Buffer, 20 mL.
8. Instructions

Other:

 Supplementary 
Sandwich
 Applicable sample 
Serum, Plasma
[Other]
The E2 quantitative test is based on a solid-phase enzyme immunoassay based on competitive binding method. A sample (serum/ plasma/urine) containing an unknown amount of E2 to beassayed (unlabeled antigen) is added to a standard amount of a conjugated E2 (labeled antigen). The labeled and unlabeled antigens are then allowed to compete for high affinity binding sites of E2 antibody on a limited number of secondary antibodiescoated on to the plate. After washing away the free antigen, the amount of labeled antigen in the sample is reversibly proportional to the concentration of the unlabeled antigen. The actual concentrations in unknown samples are obtained by means ofastandard curve based on known concentrations of unlabeled antigen analyzed in parallel with the unknowns. After washing, substrate solution is added and the enzyme is allowed to react for a fixed time before the reaction is terminated. Absorbenciesaremeasured at 450 nm using ELISA plate reader. A standard curve is produced using values from 5 standards from which absorbency values for blank tubes have been subtracted. Results for unknown may be read directly from this standard curve using eithermanual calculation or by a suitable computer program. This kit is suitable for the direct measurement of E2 in serum samples


Reference:

•  Sorderberg SF 1986 May 16(3) p419-433
•  Goodman MF 1992 Probl Vet Med 4(3) 433-444
•  Knobil, E, Rec. Prog. Horm,Res. 36: 52-88; 1980
•  Harris, G. W. and Naftolimf. The hypothalamus and control of ovulation. Brif. Med. Bullet. 26: 1-9; 1970
•  Shome, B. and Parlow, A. F. J. Clin. Endocrinol. Metabl. 39: 199-205; 1974
•  Uotila, M.; Ruoslahti, E. and Engvall,E. J. Immunol. Methods. 42: 11-15; 1981
•  Chattoraj SC 1976 NW Tietz eds, WB Saunders Chap 13, 699-823
 
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