Thyroid Tests T3 ELISA - Cosmo Bio Co.,Ltd.

Antibodies

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Thyroid Tests T3 ELISA

Catalog No.: ENC-ERKC2013
Size: 1KIT
Price: ¥125000
$1667
antigen/source: Thyroid Tests T3
Description: standard: native
catalog info: Catalog 2012-p113
Storage: 4C & -20C
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Immunogen: Canine
Reacts with: Canine
Sensitive: 0.5 ng / mL
Purpose: The T3 ELISA test is an immunoassay designed for the quantitative determination of triiodothyronine (T3) in serum/plasma samples of canine and related species.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Antibody-coated microtiter wells, 96-well plate
2. T3 HRP Conjugate, 12mL
3. TMB Color Reagent (Ready to use) 12 mL
4. Stop solution (2N HCl) 6 mL
5. 20X Wash Buffer , 20 mL
6. Reference Standard, 1 set, ready to use (0, 0.5, 1, 2.5, 5, 10, 20 ng / mL)
7. Instructions

Other:

 Supplementary 
Sandwich
 Applicable sample 
Serum, Plasma
[Other]
TEST PRINCIPLE
In the T3 ELISA Test, a certain amount of anti T3 antibody is coated on microtiter wells. A measured amount of canine serum/plasma and a constant amount ofT3 conjugated with horseradish peroxidase are added to the microtiter wells. During 2 hour incubation at 37C, the T3 and conjugated T3 compete for the limited binding sites on the anti-T3 antibody on the wells. After the incubation period, the wellsare washed 5 times with wash buffer to remove any unbound T3 conjugate. A solution of TMB substrate is then added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of stopsolution and the absorbency is measured spectrophotometrically at 450nm. The intensity of the color formed is proportional to the amount of enzyme present and is inversely related to the amount of unlabeled T3 in the sample. By reference to a seriesofT3 standards assayed in the same way, the concentration of T3 in the unknown sample is quantified.


Reference:

•  Panceiera DL, 1999 J Small Anim Pract 40 (4)p152-157
•  Dixon RM and Mooney CT, 1999 J Small Anim. Pract 40(2) P72-78
•  Bruner, JM et al., 1998 J Am Vet Med. Assoc. 15 212(10)., p1572-1575
•  Scott-Moncrieff JCet al.,1998 J Am Vet, Med. Assoc. 1;212(3):p387-391
•  Williams Daet al.,1996 J Am Vet Med Assoc. 15,209(10):p1730-1732
•  Thoresen SJet al.,1996 Vet Res Commu 20(5) p411-420
•  Walker W.H.C. Introduction: An Approach to Immunoassay. Clin. Chem. 1977; 23: 384
•  Wisdom G.B. Enzyme-Immunoassay. Clin. Chem. 1976; 22: 1243
•  Hoffenberg R. Medicine 1978; 8: 392
•  Lieblich J., Utiger R.D. J. Clin. Invest. 1972; 51: 1939
 
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