Thyroid Stimulating Hormone (TSH) ELISA - Cosmo Bio Co.,Ltd.

Antibodies

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Thyroid Stimulating Hormone (TSH) ELISA

Catalog No.: ENC-ERKC2015
Size: 1KIT
Price: ¥125000
$1667
antigen/source: Thyroid Stimulating Hormone
Description: standard: native
catalog info: Catalog 2012-p113
Storage: 4C & -20C
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Immunogen: Canine
Reacts with: Canine
Sensitive: 1 ng / mL
Purpose: The Canine TSH ELISA test is an immunoassay designed for the quantitative determination of thyroid stimulating hormone (TSH) in serum/plasma samples of Canine and related species.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Antibody-coated microtiter wells
2. Reference standard, Ready to use (0, 1, 2.5, 5, 10, 25 ng / mL), 0.6 mL / vial
3. Enzyme Conjugate Reagent, 12 mL
4. TMB color reagent (ready to use) , 12 mL
5. 20X Wash buffer, 20 mL
6. Stop solution (2N HCl), 6 mL
7. Instructions

Other:

 Supplementary 
Sandwich
 Applicable sample 
Serum, Plasma
[Other]
TEST PRINCIPLE
The Canine TSH ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes affinity purified antibodydirected against intact Canine TSH molecule for solid phase (microtiter wells) immobilization and a mouse anti bovine TSH beta antibody is in the antibody-enzyme (horseradish peroxidase) conjugate. The test sample is allowed to react simultaneouslywith the two antibodies, resulting in the TSH molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 3 hours of incubation period at 37 C, the wells are washed with wash solution to remove unbound-labeled antibodies.Asolution of TMB is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of stop solution and the color is changed to yellow and measured spectrophotometrically at 450nm.The concentration of TSH is directly proportional to the color intensity of the test sample.


Reference:

•  Panceiera DL, 1999 J Small Anim Pract 40 (4)p152-157
•  Dixon RM and Mooney CT, 1999 J Small Anim. Pract 40(2) P72-78
•  Bruner, JM et al., 1998 J Am Vet Med. Assoc. 15 212(10)., p1572-1575
•  Scott-Moncrieff JCet al.,1998 J Am Vet, Med. Assoc. 1;212(3):p387-391
•  Williams Daet al.,1996 J Am Vet Med Assoc. 15,209(10):p1730-1732
•  Walker W.H.C. Introduction: An Approach to Immunoassay. Clin. Chem. 1977; 23: 384
•  Wisdom G.B. Enzyme-Immunoassay. Clin. Chem. 1976; 22: 1243
•  Hoffenberg R. Medicine 1978; 8: 392
•  Lieblich J., Utiger R.D. J. Clin. Invest. 1972; 51: 1939
 
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