Insulin ELISA - Cosmo Bio Co.,Ltd.

Antibodies

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Insulin ELISA

Catalog No.: ENC-ERKF4009
Size: 1KIT
Price: ¥125000
$1667
antigen/source: Insulin
Description: standard: native
catalog info: 2008 Japanes Assay kit Catalog-p819
Storage: 4C
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Immunogen: Feline
Reacts with: Feline
Sensitive: 0.5ng/ml
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Antibody-coated microtiter wells, 96-well plate
2. Reference Standards: 0, 1, 2.5, 5, 10, 25ng/ml (0.5mL/Vial)
3. Enzyme Conjugate Reagent, 12 mL
4. TMB Color Reagent (ready to use) , 12 mL
5. 20X Wash buffer, 20 mL
6. Stop solution (2N HCl), 6mL
7. Instructions

Other:

 Supplementary 
Competitive
 Applicable sample 
Serum, Plasma
 Background 
Insulin is a peptide hormone very intimately involved in the control and regulation of all cellular activities of carbohydrate
homeostasis, and is secreted by the beta cells of the pancreas. The circulating sugars intern insert a feedback regulation on the
secretion of Insulin. Physiological circulating levels of Insulin is very important for many cellular, organ and body functions. Any
changes in levels leads toDiabetes and related complex cycle of pathological events.
 Principle 
The Insulin ELISA Test Kit is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes
anti-Insulin antibodies for solid phase (microtiter wells) immobilization and a mouse monoclonal antibodies in the antibody-enzyme
(horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the antibodies, resulting in
Insulin molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 60 minute incubation period, at
37C, the wells are washed with wash buffer to remove unbound labeled antibodies. A solution of TMB is added and incubated for 20
minutes, resulting inthe development of a blue color. The color development is stopped with the addition of 2N HCl, and the
absorbency is measured spectrophotometrically at 450nm. The intensity of the color formed is proportional to the amount of enzyme
present and isdirectly related to the amount of unlabeled Insulin in the sample. By reference to a series of Insulin standards assayed in
the same way, the concentration of Insulin in the unknown sample is computed and quantified.

 
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