||0.1 ng / ml.
||The Corticosterone ELISA test is an immunoassay designed for the quantitative determination of Corticosterone in serum/plasma/urine. The test is intended for professional use as an aid in the determination and monitoring of physiological/pathological conditions related to serum/plasma Corticosterone in Primates and related species.
1. Microtiter wells coated with Corticosterone specific antibody.
2. Enzyme labeled (HRP) Corticosterone solution, 12 mL
3. QC Controls: Low (1-2 ng/ml) and high range (5-10).
4. TMB Color Reagent (One-step ready to use), 12 mL
5. Stop Solution (2N HCl), 6 mL
6. 20x Wash Buffer, 20 mL.
7. Sample diluent, 25 mL
8. Corticosterone, (Standards/Calibrators) Set. (0, 0.5, 1, 2, 5, 10, 20 ng per mL) 0.5 mL / Vial
Serum, Plasma, Urine
The Corticosterone Quantitative ELISA Test is based on a widely used immunoassay technique. A sample (serum/ plasma/urine) containing an unknown amount of Corticosterone to be assayed (unlabeled antigen) is added to a standard amount of a labeled derivative of the same substance (labeled antigen). The labeled and unlabeled antigens are then allowed to compete for high affinity binding sites on a limited number of antibodies coated on to the plate. After washing away the free antigen, the amount of labeled antigen in the sample is reversibly proportional to the concentration of the unlabeled antigen. The actual concentrations in unknown samples are obtained by means of a standard curve based on known concentrations of unlabeled antigen analyzed in parallel with the unknowns. In this kit HRP enzyme label is used. The biospecific reaction takes place during 2 hour incubation at 37C. After washing away, substrate solution is added and the enzyme allowed to react for a fixed time before the reaction is terminated. Absorbencies are measured at 450 nm using ELISA plate reader. A standard curve is produced using values from 6 standards from which absorbency values for blank tubes have been subtracted. Results for unknown may be read directly from this standard curve using either manual calculation or by a suitable computer program.
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